Histopathological examination of Allolobophora calignosa efficacy against glucocorticoid-induced osteoporosis in mice
Department of Zoology, Faculty of Science, Cairo University 12613, Giza, Egypt.
Research Article
GSC Biological and Pharmaceutical Sciences, 2023, 22(01), 125-133.
Article DOI: 10.30574/gscbps.2023.22.1.0499
Publication history:
Received on 25 November 2022; revised on 09 January 2023; accepted on 11 January 2023
Abstract:
Osteoporosis is a disease characterized by low bone mass, deterioration of bone tissue, and disruption of bone microarchitecture: it can lead to compromised bone strength and increased risk of fractures. Secondary osteoporosis is mostly glucocorticoid-induced osteoporosis (GIOP). According to traditional Chinese medicine, the kidney influences bone, and bone loss are associated with kidney and liver inadequacies. We tested Allolobophora caliginosa extract (EE) and Allolobophora caliginosa coelomic fluid (CF) for anti-osteoporotic activity against GIOP. 28 male mice were separated randomly into two groups; the control received distilled water, and the second group received 1 mg/kg dexamethasone daily for 28 days. The second group was divided randomly into three groups. The first OP subgroup received distilled water orally every other day for 28 days. The other two OP subgroups received EE (45 mg/kg body weight) and CF (20 mg/kg) orally for 28 days. EE and CF preserved cortical and trabecular bone loss, decreased bone marrow space, and enhanced chondrocyte production. EE and CF maintained hepatic necrosis in the liver and decreased renal degeneration in the kidney caused by GIOP. In this study, Histological images showed that EE at 45 mg/kg was highly effective as an anti-osteoporotic against GIOP, while CF at 20 mg/kg was moderately effective.
Keywords:
Glucocorticoids; Osteoporosis; Earthworm; Antiosteoporosis
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Copyright © 2023 Author(s) retain the copyright of this article. This article is published under the terms of the Creative Commons Attribution Liscense 4.0
