Methods: This study evaluates the bioavailability of calcium from eggshell powder and its effect on ascorbic acid bioavailability. Eggshell powder was sourced, processed, and formulated with animal feed in varying ratios (3:1 and 6:1 feed-to-eggshell). Experimental groups of Swiss albino mice were treated with these formulations, vitamin C, and control diets over four weeks. Serum calcium and ascorbic acid levels were analyzed weekly.

Results: Results demonstrated that eggshell supplementation significantly increased serum calcium levels, particularly in the 3:1 formulation combined with vitamin C. Serum ascorbic acid levels were also positively influenced, with the 6:1 formulation showing cumulative benefits over time. These findings highlight the potential of eggshell powder as a cost-effective, bioavailable calcium source, enhancing nutrient absorption and overall health outcomes.