Inhibitory effects of Moringa oleifera seed extracts on crude xanthine oxidase
1 Department of Biochemistry, School of Life Science, Federal University of Technology Minna, Bosso LGA, Niger State, Nigeria.
2 Department of Chemistry and Biochemistry, Faculty of Health Science, The University of Oklahoma, Tulsa, United States of America.
Research Article
GSC Biological and Pharmaceutical Sciences, 2023, 25(03), 120–128.
Article DOI: 10.30574/gscbps.2023.25.3.0518
Publication history:
Received on 29 October 2023; revised on 12 December 2023; accepted on 15 December 2023
Abstract:
The quest for novel xanthine oxidase (XO) inhibitors, characterized by reduced side effects and heightened therapeutic efficacy, holds significant importance in addressing hyperuricemia and gout. This study was undertaken to assess the inhibitory properties of Moringa oleifera seed extracts on crude XO. The isolation of crude XO from bovine (cow) milk was achieved through ammonium sulfate precipitation techniques, and its activity was subsequently quantified using spectrophotometric measurements at a wavelength of 293 nm. The impact on xanthine oxidase inhibition was investigated utilizing varying concentrations of Moringa oleifera seed extracts in methanol, ethyl acetate, and aqueous solutions. Among these extracts, the ethyl acetate variant displayed the most robust inhibitory effect, with a notable inhibition rate of 89.16% ± 0.42, followed by the methanol extract at 65.59% ± 0.79 and the aqueous extract at 48.77% ± 0.85. For comparison, the positive control (allopurinol) exhibited an inhibition rate of 94.32% ± 0.66. The kinetic parameters of the enzyme were determined, revealing Vmax and km values of 0.05 U/ml and 131.35 µM, respectively. When the enzyme was exposed to the inhibitory extracts, the resulting Vmax and km values were as follows: ethyl acetate extract; 0.03 U/ml and 77.12 µM, methanol extract; 0.04 U/ml and 131.35 µM, and aqueous extract; 0.02 U/ml and 74.23 µM. This research work establishes a foundation for the potential utilization of Moringa oleifera seed extracts in addressing hyperuricemia and, consequently, gout.
Keywords:
Xanthine oxidase; Moringa oleifera; Inhibition; Hyperuricemia
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Copyright © 2023 Author(s) retain the copyright of this article. This article is published under the terms of the Creative Commons Attribution Liscense 4.0
