Isolation and characterization of chemical constituents, cytotoxicity, antibacterial and antioxidant activity of the isolates, crude extract from Hornstedtia scyphifera var leaf
1 Department of Biochemistry, Federal University Wukari Taraba State.
2 Department of Biochemistry, Modibbo Adama University of Technology Yola Adamawa State.
3 Department of Biochemistry, University of Maiduguri Borno State.
Research Article
GSC Biological and Pharmaceutical Sciences, 2020, 10(02), 070-098.
Article DOI: 10.30574/gscbps.2020.10.2.0027
Publication history:
Received on 01 February 2020; revised on 06 February 2020; accepted on 12 February 2020
Abstract:
Hornstedtia scyphifera var belongs to ginger family, they are most of time used as spices for cooking, preservatives in food industry and colouring agents. It provides health-promoting effects to cutile certain diseases and ailment such as stomach-ache, Catarrh, asthma, diarrhoea, cold and cough, digestive disorder and rheumatism. In this study, we isolated phytochemicals and investigated the potentials of the leaf crude extract of hexane, dichloromethane, chloroform, ethyl acetate and methanol, cytotoxicity, antioxidant and antibacterial activity. The experiment was performed and two phytochemicals (quercetin and Dodecanoic acid) were isolated and characterised. Significant result was obtained from the isolated compounds and solvent crude extract with hexane and methanol have higher cytotoxicity of 35.462 and 34.059, chloroform and methanol with higher antioxidant of 34.46±0.32 and 35.33±0.210. There was an observed significant activity at 500 µg/mL in all the extracts against all the selected pathogen with zone of growth inhibition ranging from 07.70 + 27.30 mm to 27.30 ± 0.10 mm in all the solvent extract. The result indicated that with the isolation of quercetin and dodecanoic acid from Hornstedtia scyphifera var Leaf extract is a potential plant medicine which can be harnessed as an agent for antioxidant and potential antibacterial. The compound was isolated for the first time from Hornstedtia scyphifera var.
Keywords:
Isolation; Characterization; Constituents; Cytotoxicity; Antibacterial; Antioxidant; Hornstedtia scyphifera; GCMS; NMR; FTIR
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Copyright © 2020 Author(s) retain the copyright of this article. This article is published under the terms of the Creative Commons Attribution Liscense 4.0
