Microbial quality of five selected commercially herbal formulations sold in Anambra State, Nigeria

Chinedu Joseph Ikem 1, 2, *, Emmanuel Atada 1, Nnamdi Angus Oli 2, Chinemerem David Nwobodo 3, Chiagozie Malachy Ugwu 2 and Okechukwu Charles Esimone 2

1 Department of Pharmaceutical Microbiology & Biotechnology, Madonna University, Elele, River State, Nigeria.
2 Department of Pharmaceutical Microbiology & Biotechnology, Nnamdi Azikiwe University, Awka, Nigeria.
3 Department of Microbiology, Renaissance University, Enugu, Nigeria.
 
Research Article
GSC Biological and Pharmaceutical Sciences, 2022, 21(03), 134-139.
Article DOI: 10.30574/gscbps.2022.21.3.0480
Publication history: 
Received on 06 November 2022; revised on 17 December 2022; accepted on 22 December 2022
 
Abstract: 
The use of Herbal formulation has become a mainstay in developing countries in the last few decades with Nigeria embracing these practices due to the high cost and accessibility of modern medicine.
This study aimed to assess five (5) commercially available herbal formulations sold in Anambra State for their microbial quality using the pour plate method. The isolates were identified based on their morphological, macroscopic, and biochemical characteristics.
A total of five (5) herbal formulations were screened and various degrees of microorganisms were isolated ranging from Staphylococcus aureus, Staphylococcus epidermidis, and Proteus sp. For bacteria. Aspergillus niger, A. flavus, Mucor sp, Rhizopus sp, Candida sp, and Trichophyton rubrum for fungi isolates. The total bacteria count ranged from 1.6 × 103 to 5.0 × 103 CFU/mL and the total fungal count ranging 1.6 × 103 to 2.2 × 104 CFU/mL respectively.
However, it can be confirmed the microbial load is within the World Health Organization safety limit for good manufacturing practices of herbal formulation.
 
Keywords: 
World Health Organization; Herbal formulations; Anambra State; Biochemical; Microbial
 
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