Phytochemical analysis, antioxidant and anti-inflammatory activities of Chassalia kolly leaves extract, a plant used in Benin to treat skin illness
1 Laboratory of Study and Research in Applied Chemistry, Polytechnic School of Abomey-Calavi, University of Abomey-Calavi, BP 2009 Abomey-Calavi, Benin, West Africa.
2 Laboratory of Organic Chemistry and Biochemistry of the Faculty of Sciences and the Environment of the Dunarea de Jos University of Galati, Domneasca Street 47, 800008 - Galati, Romania.
Research Article
GSC Biological and Pharmaceutical Sciences, 2021, 15(03), 063–072.
Article DOI: 10.30574/gscbps.2021.15.3.0148
Publication history:
Received on 28 April 2021; revised on 30 May 2021; accepted on 04 June 2021
Abstract:
In the last decades, it has been intensively studied natural alternatives to synthetic products. Chassalia kolly is one of the medicinal plants used traditionally for treatment of skin infections. This study aimed at identifying the diversity secondary metabolites, to investigate the antioxidant and anti-inflammatory activities of ethanolic extract of Chassalia kolly leaves. Total phenols were determined by Folin Ciocalteu method. The aluminum trichloride method has been used to quantify total flavonoids, while the determination of condensed tannins was carried out by the hydrochloric vanillin method. The antioxidant capacity was evaluated by 2, 2-diphenyl-l-picrylhydrazyl (DPPH), 2, 2'-Azino-bis- (3-ethylbenzothiazolin-6-sulfonic acid) (ABTS) and phosphomolybdenum methods. In vitro anti-inflammatory activity of ethanolic extract of Chassalia kolly leaves was evaluated by method membrane stabilization. In this results, Chassalia kolly leaves contain flavonoid, mucilages, anthocyans, sterols and terpenes, saponosides, alkaloids and reducing compounds. The contents of total phenols, total flavonoids and total tannins are respectively (20.41±2.11) µgGAE/mgDM; (30.29±2.18) µgQE/mgDM and (1.211±0.122)µgCE/mgDM. The results obtained during this work allowed us to assert that all extracts of the studied plant have very good antioxidant properties (IC50=0.05µg/µL). The ethanolic extract of the leaves of Chassalia kolly showed an anti-inflammatory activity more interesting than aspirin which is the reference compound used in this study.
Keywords:
Skin; Chassalia kolly; secondary metabolies; antioxidant; anti-inflammatory
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