Phytochemical studies of Cissus populnea in Benue State, Nigeria
1 Department of Forest Production and Products, University of Agriculture, Makurdi, Nigeria.
2 Center for Agrochemical Technology, Department of Chemistry, Federal University of Agriculture Makurdi, Nigeria.
Research Article
GSC Biological and Pharmaceutical Sciences, 2018, 02(01), 009–015
Article DOI: 10.30574/gscbps.2018.2.1.0022
Publication history:
Received on 21 September 2017; revised on 27 November 2017; accepted on 08 January 2018
Abstract:
Phytochemical studies of Cissus populnea in Benue State Nigeria was carried out to determine the presence of different chemicals in the stem, based on locality. The state was stratified into three zones using the already existing political grouping. From each of the zones samples of plants were collected with a minimum of 100 m apart. This is to avoid collection from the same siblings. The collected samples were air dried and pounded to paste. The obtained paste was soaked in hexane (150 ml), ethyl acetate (150 ml) and methanol (150 ml) for 48 h each. The mixtures were agitated every 4 h and then filtered in glass jars (1 L) representing the locations for each zone. The filtrates were left open to evaporate to dryness. After that, extracts were transferred into vials. The ethyl acetate extracts were coded for identification and sent for GC-MS analysis. The results obtained confirmed the presence of different chemicals in the stem of the plants and there was variation in the type of chemical obtained based on location.
Keywords:
Phytochemical; Ethyl acetate; GC-MS; Cissus populnea
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