Rapid, simple and validated reverse phase method for analysis of glimepiride from capsules
1 Department of Chemistry, B. N. N. College, Bhiwandi, Thane, India.
2 Inventia Healthcare Pvt. Ltd., Thane, India.
3 Department of Chemistry, Ramnarain Ruia College, Mumbai, India.
Short Communication
GSC Biological and Pharmaceutical Sciences, 2018, 05(03), 043–045.
Article DOI: 10.30574/gscbps.2018.5.3.0132
Publication history:
Received on 05 November 2018; revised on 28 November 2018; accepted on 10 December 2018
Abstract:
Glimepiride, a blood sugar controlling molecule have been analyzed from a marketed capsule consisting of glimepiride and pioglitazone using a reversed phase isocratic method. The method was developed on 150 mm × 4.6 mm, 5 µ C18 column. The mobile phase consisted of sodium dihydrogen o-phosphate pH 6.0: acetonitrile (60:40 v/v). The compounds gave a good UV response at 220 nm, hence the detection wavelength. The method was found to be highly specific for glimepiride in presence of Pioglitazone and other constituents of the capsule. The developed method is simple, precise, robust and stands validated as per ICH guidelines.
Keywords:
Glimepiride; Anti-diabetic; Reverse phase; Liquid chromatography
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Copyright © 2018 Author(s) retain the copyright of this article. This article is published under the terms of the Creative Commons Attribution Liscense 4.0
