Reliability of PSA assays: A key asset for early prostate cancer detection

Asmae Rhoubi 1, 2 *, Ismail Faiz 1, 2, fatima zahra Joudar 1, 2, El Houcine Sebbar 1, 2, Dounia El Moujtahid 1, 2 and Mohamed Choukri 1, 2

1 Biochemistry Unit, Faculty of Medicine and Pharmacy Oujda, Hay AL Hikma BP Box 4867 Oujda University, 60049,
Oujda, Morocco.
2 Laboratory of Biochemistry, Mohammed VI University Hospital, Oujda, Morocco.
 
Research Article
GSC Biological and Pharmaceutical Sciences, 2025, 30(02), 001–006.
Article DOI: 10.30574/gscbps.2025.30.2.0043
Publication history: 
Received on 23 December 2024; revised on 31 January 2025; accepted on 02 February 2025
 
Abstract: 
Introduction: Prostate cancer requires early detection to reduce mortality, with PSA serving as a key marker since 1987. This assay, crucial for distinguishing benign conditions from cancer, requires rigorous precision in the laboratory. The aim of this study was to verify the method of PSA measurement in the biochemistry laboratory of the CHU Mohammed VI d'Oujda.
Materials and methods: This prospective study, conducted at the CHU Mohammed VI d'Oujda over 30 days, assessed the reliability of total and free PSA measurements on the Abbott Alinity ci® analyzer. It assessed reproducibility through multi-operator tests and repeatability through consecutive analyses of standardized samples. The CMIA method enabled rigorous quantitative measurement, with analysis of coefficients of variation compared to CLSI standards to guarantee precision and accuracy, validated by intermediate management software.
Results: The study assessed the repeatability and reproducibility of total and free PSA assays at different concentration levels. The results indicate that the majority of values fall within the acceptable range of variation ([-1S, 1S]), although minor variations were observed depending on levels and handling conditions. These results confirm the accuracy of the assay methods and underline their reliability for robust clinical analyses.
Discussion and conclusion: Our results demonstrate good repeatability and reproducibility of tPSA and fPSA assays on Abbott's Alinity ci® analyzer, in line with performance standards established in the literature.
 
Keywords: 
Early detection; Prostate cancer; PSA; Repeatability; Reproducibility
 
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Copyright information: 
Copyright © 2025 Author(s) retain the copyright of this article. This article is published under the terms of the Creative Commons Attribution Liscense 4.0

 

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