Simplex Immunodotblot for detecting dog meat contaminants in foodstuffs
Faculty of Animal Science, University of Mataram, Jl. Majapahit No. 62, Mataram 83125 - NTB, Indonesia.
Research Article
GSC Biological and Pharmaceutical Sciences, 2024, 29(03), 386-389.
Article DOI: 10.30574/gscbps.2024.29.3.0368
Publication history:
Received on 03 October 2024; revised on 16 December 2024; accepted on 18 December 2024
Abstract:
This study is an initial effort to develop a prototype kit based on the simplex immunodot blot method to detect dog meat contamination in food products. The simplex immunodotblot is an immunoassay technique that identifies specific proteins or antigens through antibody-antigen interactions, targeting a single contaminant in one reaction. The antigen utilized was dog meat extract (DME) prepared in the laboratory, while the primary antibodies (Ab) against DME were produced in-house using laying hens. Test samples were applied in varying concentrations (5, 10, and 20 µg per spot) onto comb-shaped polyester plastic sheets. A commercial secondary antibody against IgY, labeled with horseradish peroxidase (HRP), served as the detector. The reaction was visually inspected; in cases where dog meat was present, a blue or black spot appeared due to the reaction between the antigen, antibody, enzyme, and substrate. The study demonstrated the successful development of the simplex immunodotblot, which was able to detect dog meat visually at concentrations as low as 5 µg in a laboratory setting. However, further research is required, particularly to adapt the prototype kit for halal food authentication purposes.
Keywords:
Antibody; Beef; Dog meat; Halal; Immunodotblot; Meat extract
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Copyright © 2024 Author(s) retain the copyright of this article. This article is published under the terms of the Creative Commons Attribution Liscense 4.0
