Simultaneous quantitative estimation of ellagic acid and gallic acid in Sudanese Solanum dubium seed by high performance thin layer chromatography (HPTLC)
1Department of Chemistry, Preparatory College, University of Medical Sciences and Technology, Khartoum Sudan.
2 Natural Product Laboratory, Faculty of Pharmacy, Hamdard University, Hamdard Nagar, New Delhi 110062,
3 Department of Pharmacology, Faculty of Pharmacy, National Ribat, University, P.O. Box 2404 Khartoum Sudan.
4Department of Pharmacognosy, Faculty of Pharmacy, University of Medical Sciences and Technology, P.O. Box 12810, Khartoum, Sudan.
Research Article
GSC Biological and Pharmaceutical Sciences, 2020, 13(01), 054-061.
Article DOI: 10.30574/gscbps.2020.13.1.0311
Publication history:
Received on 25 September 2020; revised on 07 October 2020; accepted on 12 October 2020
Abstract:
Background: Solanum dubium is a plant believed to have several therapeutic effects including anti-asthmatic properties. The objective of this study was to investigate the quantitative estimation of Gallic acid and Ellagic acid from the seed extract of Sudanese Solanum dubium.
Methods: A simple and rapid high-performance thin-layer chromatographic method was developed and validated for quantitative estimation of Gallic acid and Ellagic acid from the seed extract of Sudanese Solanum dubium.
Results: Ellagic acid and Gallic acid were quantified by using HPTLC. The seeds were found to contain 1.1% w/w of Ellagic acid and 2.1% w/w of Gallic acid in extract. Gallic acid and Ellagic acid were chromatographed on silica gel 60 F254 TLC plate using Toluene: Ethyl acetate – Methanol – Formic acid (3:3:1:0.4 v/v/v/v) as mobile phase and quantified by densitometric scanning at 280 nm. The method was found to give compact spots for Gallic acid (Rf 0.35)
and Ellagic acid (Rf 0.21). The linear regression analysis data for standard Gallic and Ellagic acids spots showed good linear relationship with r2 = 0.988 and 0.994 respectively, in the concentration range 100-3000 ng/spot, accurate (99.23-102.7%), (98.7-100.2%); precise (% RSD ≤ 2); robust (% RSD ≤ 2) and specific. The LOD and LOQ of the method were found as 653 and 396.8ng/spot and 215.5and 130 ng/spot, of Gallic acid and Ellagic acid respectively.
Conclusion: The method was validated for precision, recovery and repeatability as per the International Conference on Harmonization Guidelines for Gallic acid and Ellagic acid. Statistical analysis of the data showed that the method is precise, accurate, reproducible and selective for the analysis of Gallic acid and Ellagic acid.
Keywords:
Sudanese Solanum dubium seed; Gallic acid; Ellagic acid; HPTLC.
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