Validated HPTLC method development, comparative evaluation and stability study for berberine in herbal preparations
1 Department of Quality Assurance, Vidya Bharathi College of Pharmacy, Amravati Maharashtra India 444602
2 Deparment of Pharmaceutical Chemistry, Vidya Bharathi College of Pharmacy, Amravati Maharashtra India 444602
Research Article
GSC Biological and Pharmaceutical Sciences, 2024, 27(02), 281–296.
Article DOI: 10.30574/gscbps.2024.27.2.0186
Publication history:
Received on 05 April 2024 revised on 22 May 2024; accepted on 24 May 2024
Abstract:
The study focusing on the development and validation of a high-performance thin-layer chromatography (HPTLC) method for quantifying berberine in pharmaceutical formulations. Various chromatographic parameters were optimized to ensure precise and reproducible results, including mobile phase composition, linearity range, detection wavelength, and spot characteristics. The method demonstrated high accuracy and linearity within the concentration range of 5 - 35 µg/mL, with a determined Rf value of 0.52±0.02 for berberine. Additionally, stability studies revealed that berberine underwent degradation under all stress conditions examined.
Keywords:
HPTLC; Berberine; Stability Study; Quantitative Evaluation; Qualitative evaluation.
Full text article in PDF:
Copyright information:
Copyright © 2024 Author(s) retain the copyright of this article. This article is published under the terms of the Creative Commons Attribution Liscense 4.0
