In vitro callus induction and indirect organogenesis of Mentha piperita (L.) - an aromatic medicinal plant
1 Department of Botany, Faculty of Bio-Sciences, University of Barisal, Barisal-8200, Bangladesh.
2 Department of Botany, University of Rajshahi, Rajshahi-6025, Bangladesh.
Research Article
GSC Biological and Pharmaceutical Sciences, 2018, 04(03), 049–060
Article DOI: 10.30574/gscbps.2018.4.3.0078
Publication history:
Received on 10 August 2018; revised on 24 August 2018; accepted on 27 August 2018
Abstract:
The effect of different concentrations and combinations of plant growth regulators were tested for in vitro callus induction and indirect plant regeneration of Mentha piperita L. from young leaves and internodal explants. Greenish, organogenic and nodular nature of callus was achieved only from leaf disc explant when cultured on MS medium containing 1.5 mg/l NAA+ 0.2 mg/l BAP and then subsequent plant regeneration was obtained on MS medium supplemented with 2.0 mg/l BAP+0.5mg/l NAA from leaf derived callus. The highest frequency of shoot regeneration (90%) with the maximum number of shoots (15.1±0.11) was achieved in this medium. In vitro raised microshoots showed 90% response with maximum number of roots (16.3±0.23) on 1.0 mg/l IBA after 11-12 days. Then in vitro rooted plantlets were move to polybags bearing soil and vermiculite mixture in 1:1 ratio for hardening and hardened plantlets showed 95% survivability on field conditions.
Keywords:
In vitro; Callus induction; Organogenesis; Mentha piperita; Vermiculite
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Copyright © 2018 Author(s) retain the copyright of this article. This article is published under the terms of the Creative Commons Attribution Liscense 4.0.
