The antispasmodic activity of ethanol extract of the stem bark of Piliostigma reticulatum Horscht D.C (Ceasalpiniaceae), and its dichloromethane fraction isolated
1 Laboratory of Physiology and Pharmacology, UFR Environment, University of Jean Lorougnon Guédé of Daloa, P.O 02 Box 150 Daloa 02, Daloa, Côte d'Ivoire.
2 Laboratory of Biotechnology in agro-resources valorization and natural substances, UFR Biological Sciences, University of Korhogo, P.O Box 1328 Korhogo, Korhogo, Côte d'Ivoire.
3 Department of Pharmacology and Toxicology, School of Pharmacy, College of Health Sciences, University of Ghana, P.O Box KB 52, Korle-Bu, Accra, Ghana.
4 Laboratory of Physiology and Pharmacology, UFR Natural Sciences, University of Nangui Abrogoua, 01 P.O Box 801 Abidjan 01, Abidjan, Côte d'Ivoire.
Research Article
GSC Biological and Pharmaceutical Sciences, 2020, 11(01), 036-045.
Article DOI: 10.30574/gscbps.2020.11.1.0049
Publication history:
Received on 25 February 2020; revised on 02 April 2020; accepted on 04 April 2020
Abstract:
Piliostigma reticulatum (Caesalpiniaceae) has been used in traditional medicine for the treatment of gastrointestinal disorders like diarrhea. The aim of the present study was to examine the relaxant activity of an extract of P. reticulatum stem bark and five of its fractions on isolated rabbit duodenum. Segments of duodenum of rabbits were suspended in an organ bath. The ethanol extract had been fractioned to obtain some fractions heptane, dichloromethane, ethyl acetate, butanol and aqueous extract. The ethanol extract and the dichloromethane fraction were tested on the contraction of rabbit duodenum at the increasing concentrations. Increasing concentrations of P. reticulatum ethanol extract and its fraction induced a relaxant effect on spontaneous rabbit duodenum. The ethanol extract reduced duodenum contraction totally at 1.32 mg/mL at IC50 = 0.62 mg/mL. At the test concentration of 0.52mg/mL in organ bath, the dichloromethane fraction showed a relaxation of 100% (IC50 = 0.24 mg/mL). Piliostigma reticulatum stem bark possesses antispasmodic properties that can at least explain and support its traditional use against diarrhea.
Keywords:
Antispasmodic activity; Piliostigma reticulatum; Gastrointestinal disorders.
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