Two antihyperglycaemic compounds from Globimetula braunii (Engl.) Van Tiegh (Loranthaceae)
1Department of Pharmacognosy, Obafemi Awolowo University, Ile-Ife, Osun State, Nigeria.
2 Drug Research and Production Unit, Obafemi Awolowo University, Ile-Ife, Osun State, Nigeria.
3 Department of Chemistry, Obafemi Awolowo University, Ile-Ife, Osun State, Nigeria.
Research Article
GSC Biological and Pharmaceutical Sciences, 2020, 11(01), 046-054.
Article DOI: 10.30574/gscbps.2020.11.1.0066
Publication history:
Received on 13 March 2020; revised on 25 March 2020; accepted on 02 April 2020
Abstract:
Large numbers of medicinal plants are constantly being screened for possible pharmacological values, especially for chronic diseases such as diabetes, with the view of discovering new compounds that may serve as templates for synthesis of more active/less toxic drugs. Therefore, the antihyperglycaemic activity of the leaf of Globimetula braunii with antidiabetic ethnomedical usage in Nigeria was investigated is this study to justify this folkloric claim. The median lethal dose, LD50 of the ethanol leaf extract of G. braunii was determined using Lorke’s method and its antihyperglycaemic effect at 100, 200 and 400 mg/kg was evaluated using glucose-induced hyperglycaemic rats while glibenclamide (5 mg/kg) and 1 % Tween 80 in normal saline served as positive and negative controls, respectively. Anti-hyperglycaemic activity-directed purification of the extract of the plant in glucose-loaded rats, led to the isolation and characterisation of phyllanthone and methyl 2, 6-dihydroxy-4-methoxybenzoate from the dichloromethane partitioned fraction. The findings showed that the LD50 of the ethanol leaf extract of G. braunii was greater than 5,000 mg/kg while its 100 mg/kg was the most active dose with comparable activity (p>0.05) to the standard drug, glibenclamide. The dichloromethane and aqueous partitioned fractions of the extract were the most promising fractions. Chromatographic separations of the dichloromethane fraction yielded phyllanthone, and methyl 2, 6-dihydroxy-4-methoxybenzoate that elicited comparable activity to glibenclamide (5 mg/kg) at 10 and 20 mg/kg at all time-points. The study justified the antidiabetic folkloric use of G. braunii leaf and confirmed phyllanthone and methyl 2,6-dihydroxy-4-methoxybenzoate as two of its antihyperglycaemic constituents.
Keywords:
Globimetula braunii; Antihyperglycaemic activity; Phyllanthone; methyl 2,6-dihydroxy-4-methoxybenzoate
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