Concurrent analysis of ambroxol HCl and salbutamol sulphate from tablet formulation by RP-HPLC
1 IBSS’s Dr. Rajendra Gode Institute of Pharmacy, Mardi Road, Amravati-444 602, MS, India.
2 IBSS’s Dr. Rajendra Gode College of Pharmacy, Mardi Road, Amravati-444 602, MS, India.
Research Article
GSC Biological and Pharmaceutical Sciences, 2020, 13(03), 197-202.
Article DOI: 10.30574/gscbps.2020.13.3.0404
Publication history:
Received on 08 December 2020; revised on 15 December 2020; accepted on 17 December 2020
Abstract:
RP-HPLC method was developed for concurrent analysis of ambroxol HCl and salbutamol sulphate from tablet formulation. Analytes were separated with mobile phase consisting of mixture of methanol and water (0.1% triethylamine) in the ratio 50: 50 at a flow rate of 0.7 ml/min with Nucleosil (4.6 mm I.D x 250 mm) C18 column. The retention time of ambroxol HCl and salbutamol sulphate was found to be 3.61 and 6.20 min, respectively. The detection was carried out at 224 nm. The dynamic range for ambroxol HCl and salbutamol sulphate observed was 15-75 µg/ml and 1-5 µg/ml, respectively. The percent recovery obtained for ambroxol HCl and salbutamol sulphate were close to 100%. Obtained statistical data of results was found to satisfactorily.
Keywords:
RP-HPLC; Ambroxol HCl; Salbutamol sulphate; Validation
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