Ethanolic extract of Salacca zalacca peel reduce IL-1β and apoptosis in high glucose induced zebrafish embryo
1 Department of Pharmacology, Faculty of Medicine, Universitas Brawijaya, Malang, Indonesia.
2 Master Program of Biomedical Sciences, Faculty of Medicine, Universitas Brawijaya, Malang, Indonesia.
3 Department of Neurology, Faculty of Medicine, Universitas Brawijaya, Malang, Indonesia.
4 Department of Nutrition, Faculty of Medicine, Universitas Brawijaya, Malang, Indonesia.
Research Article
GSC Biological and Pharmaceutical Sciences, 2021, 16(03), 024–033.
Article DOI: 10.30574/gscbps.2021.16.3.0213
Publication history:
Received on 24 July 2021; revised on 28 August 2021; accepted on 30 August 2021
Abstract:
Gestational diabetes is a glucose intolerance level first identified during pregnancy. This condition is caused by hormone secretion that hinders insulin action from the placenta which caused insulin resistance that will cause hyperglycemia. This condition will increase oxidative stress, and in consequence increasing inflammation and cell death which can hinder fetal growth and development. Salacca zalacca peel is a food waste that contains antioxidants and anti-inflammatory properties. This research aimed to study the effect of SZ peel ethanolic extract in the expression of IL-1β and cell death marker (BAX and Apaf-1) in glucose 3% induced zebrafish embryo. Glucose metabolism is measured by the level of Phosphoenolpyruvate carboxykinase (PEPCK) at the age of 3 dpf. The expression of PEPCK, IL-1β, Bax, and Apaf-1 is measured using reverse transcriptase PCR (RT-PCR) method. Salacca zalacca peel extract is given in the concentration of 0,1; 0,2; and 0,4 mg/mL. The result of this research proves that PEPCK increases in the group which was given glucose 3%. Salacca zalacca peel extract significantly decreases the expression of IL-1β and Bax in the concentration of 0,4 mg/mL, and also able to decrease Apaf-1 expression with the result that most closely resemble the negative control in the concentration of 0,4 mg/mL. In conclusion, Salacca zalacca peel extract protects from gestational diabetes condition through the decrease of IL-1β, Bax, and Apaf-1.
Keywords:
Glucose; Salacca zalacca; PEPCK; IL1-β; BAX; Apaf-1
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Copyright © 2021 Author(s) retain the copyright of this article. This article is published under the terms of the Creative Commons Attribution Liscense 4.0
