Detection of antibiotic resistance in bacteria isolated from industrial sewage

Nseabasi-Maina N *, Agwa OK and Okpokwasili GC

Department of Microbiology, University of Port Harcourt, Rivers State, Nigeria.
 
Research Article
GSC Biological and Pharmaceutical Sciences, 2022, 18(02), 058–065.
Article DOI: 10.30574/gscbps.2022.18.2.0306
Publication history: 
Received on 09 September 2021; revised on 12 October 2021; accepted on 14 October 2021
 
Abstract: 
Industrialization and indiscriminate discharge of sewage being implicated in environmental pollution is responsible for the release of micropollutants which over time is expected to alter the physiology and metabolic pathways of microbiota native to the environment. This study was undertaken to track antibiotic resistant genes in industrial wastewater. Heterotrophic bacterial count indicated a vast abundance of culturable bacteria (76-232.66× 104 Cfu/ml). Hydrocarbon utilizing bacteria count ranged between (91.66 - 111.6×104 Cfu/ml). Antibiotic resistant bacteria isolates from industrially impacted wastewater identified by Sanger’s sequencing included Bacillus lichenforminis (KF737353.1), Alkanindiges sp. 5-0-9 (LT158291.1), Bacillus thuringiensis (MK875170.1, MK517632.1 EU697392.1) Bacillus altitudinis (KY777585.1), Bacillus cereus (KR185830.1) and Bacillus subtilis (MK124647.1). This study infers that industrial effluent is a potent reservoir for antibiotic resistant bacteria of environmental and public health concerns.
 
Keywords: 
Antibiotic; Resistance; Bacteria; Sewage
 
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