Performance of UMELISA SARS-CoV-2 antigen and STANDARD F COVID-19 in the diagnosis of COVID-19 in Villa Clara, Cuba
1 Provincial Laboratory of Microbiology and Sanitary Chemistry, Provincial Center of Hygiene, Epidemiology and Microbiology (CPHEM), Villa Clara, Cuba.
2 Academic Area of Health, Maya World University, México.
3 Mariana Grajales" Obstetrics and Gynecology Hospital, Villa Clara, Cuba.
4 Faculty of Health Technology and Nursing (FTSE), University of Medical Sciences of Villa Clara (UCM-VC), Cuba.
5 Central University "Marta Abreu" of Las Villas, Villa Clara, Cuba.
Research Article
GSC Biological and Pharmaceutical Sciences, 2022, 19(03), 279–286.
Article DOI: 10.30574/gscbps.2022.19.3.0251
Publication history:
Received on 19 May 2022; revised on 23 June 2022; accepted on 25 June 2022
Abstract:
A cross-sectional descriptive study was carried out with the objective of describing the performance of UMELISA SARS-CoV-2 Antigen and STANDARD F COVID-19 Ag FIA in the diagnosis of COVID-19, at the Provincial Laboratory of Microbiology and Sanitary Chemistry of Villa Clara, during the month of February 2022. 1056 patients who underwent Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR) using the QIAcube HT genome extraction technique, UMELISA SARS-CoV-2 Antigen and STANDARD F COVID-19 Ag FIA were included. Sensitivity, specificity, positive and negative predictive value were calculated as efficacy indicators and Spearman's Rho coefficient for the correlation between variables. The result was that 29.3% were sick, of which 189 were female, with a median age of 46 years. 74.8% had symptoms, most often fever, cough and runny nose. UMELISA SARS-CoV-2 Antigen showed higher specificity with 97.45%; while STANDARD F COVID-19 Ag FIA had higher sensitivity with 99.35%. It was shown that there is a significant inverse correlation between the RT-PCR cycle threshold and the fluorescence values of both techniques studied (p=0.000). About a third of the patients studied were sick with symptoms, with a predominance of females. UMELISA SARS-CoV-2 Antigen turned out to be more useful in confirming the presence of the disease, while STANDARD F COVID-19 Ag FIA showed better performance in ruling out the presence of the disease.
Keywords:
Performance; UMELISA SARS-Cov-2 Antigen; STANDARD F COVID-19 Ag FIA; RT; PCR; COVID-19
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Copyright © 2022 Author(s) retain the copyright of this article. This article is published under the terms of the Creative Commons Attribution Liscense 4.0
