Isolation and identification of microorganisms associated with Jollof rice sold at Bukateria in Obafemi Awolowo University, Ile -Ife, Osun State, Nigeria
Department of Microbiology, Faculty of Science, Obafemi Awolowo University, Ile-Ife, Osun State, Nigeria.
Research Article
GSC Biological and Pharmaceutical Sciences, 2023, 22(01), 178-185.
Article DOI: 10.30574/gscbps.2023.22.1.0274
Publication history:
Received on 02 July 2022; revised on 12 January 2023; accepted on 15 January 2023
Abstract:
This study assessed the microbiological quality of Jollof rice by isolating, identifying and characterizing the microorganisms associated with jollof rice. Six replicate samples were collected from different locations in Obafemi Awolowo University campus, Ile-Ife, Osun-State, Nigeria. Maconkey, Nutrient and Potatoes Dextrose agars were used for the isolation and determination of microbial load. Standard morphological and biochemical tests were carried out for the identification and characterization of isolates. A total of 10 bacteria and 10 fungal species were isolated. The total bacterial count ranged from 3.6×103cfu/g to 1.54×105cfu/g, while the total fungal count ranged from 1.04×104sfu/g to 3.0×105sfu/g. The organisms presumptively identified with the percentage occurrence include; Staphylococcus saprophiticus (20%), Proteus vulgaris (10%), Bacillus subtlis (10%), Proteus mirabilis (10%), Micrococcus varians (10%), Bacillus licheniformis (10%), Bacillus cereus (10%), Bacillus polymyxa (10%), Micrococcus luteus (10%) for bacteria isolates and Aspergillus niger (30%), Aspergillus oryzae (30%) and Aspergillus candidus (40%) for fungi isolates. Good personal hygiene, proper sanitation practice and the use of clean utensils during food preparation are recommended to avoid food poisoning and spoilage.
Keywords:
Food Safety and Hygiene; Foodborne organisms; Contamination; Jollof Rice
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Copyright © 2023 Author(s) retain the copyright of this article. This article is published under the terms of the Creative Commons Attribution Liscense 4.0
