Determination of the antioxidant property from the flavonoid rich subextract of Clitoria ternatea

owel Paningbatan Catchillar 1, 2, 3, 4, Jan Karlo Tiongson Ecalne 1, 5, 6, Juliann Marie Abaday Cayaba 5, Claire Noelle Piit Aguipo 5, Rhisand Marie Rosallo Gadian 5, Anne Kathleen Villaseran Osia 5, Bridget Orillaneda Saludes 5, Danica Jen Tagapan Soria 5, Renil Phi Venice Saligumba Torion 5, Sandra Montemayor Valentin 3, 7, Sandra Enriquez Laguimun 1, 6, Jan Ken Emmanuel Tiongson Ecalne 1, Mylene Sevilla Andal 1 and Saffia Anzures Abu-Shendi 1

1 School of Pharmacy, Centro Escolar University, 9 Mendiola St., San Miguel, Manila, Philippines 1005.
2 School of Pharmacy, Far Eastern University, Regalado Ave., West Fairview Quezon City, Philippines 1118.
3 College of Pharmacy, Virgen Milagrosa University, Dr. Martin P. Posadas Avenue, San Carlos City, Pangasinan, Philippines 2420.
4 School of Pharmacy, Philippine Women’s University, 1743 Taft Avenue, Manila Philippines 1004.
5 Allied Health Program, Lourdes College, Cagayan De Oro City, Philippines 9000.
6 College of Pharmacy, Adamson University, Ermita, Manila, Philippines 1000.
7 School of Advanced Studies, Saint Louis University, Baguio City, Philippines 2600
 

 

Research Article
GSC Biological and Pharmaceutical Sciences, 2023, 23(02), 142-147.
Article DOI: 10.30574/gscbps.2023.23.2.0201
Publication history: 
Received on 12 April 2023; revised on 19 May 2023; accepted on 21 May 2023
 
Abstract: 

This study evaluated the phytochemical, total phenolic and flavonoid content, and antioxidant property of the Clitoria ternatea flower extract using, 2-diphenyl-1picrylhydrazyl (DPPH) free radical scavenging activity. Clitoria ternatea flower sample were collected from Herbanext Laboratories Inc., was oven dried at 60oC for 6hrs, then macerated using 70% ethanol and concentrated using a rotary evaporator at 600C to obtain a syrupy crude extract, the sample was subjected to phytochemical screening to check for the presence of flavonoids and phenols, and was further used for sub-extraction using water, n-hexane and ethyl acetate as partitioning solvents and analyzed for its total phenolic and flavonoid content. The sub-extract with the highest concentration of phenols and flavonoids were then subjected to DPPH free radical scavenging activity. The results revealed that among the 3 sub-extracts subjected to total phenolic and total flavonoid content, the ethyl acetate (174.60 ± 5.32 mg GAE/g & 347.27 ± 8.79 mg QE/g) sub-extract has the highest concentration, compared to the aqueous (42.34 ± 1.84 mg GAE/g & 29.49 ± 5.28 mg QE/g) and n-hexane (15.14 ± 0.99 mg GAE/g & 272.42 ± 2.29 mg QE/g) sub-extracts, the flavonoid rich sub-extract was then subjected to antioxidant assay using the DPPH free radical scavenging activity, the result showed that the extract exhibits concentration dependent activity with an IC50 value of 75.13 ± 3.14 ppm compared to the standard gallic acid (1.74 ppm). Further investigation using different parts of the plant and various purification methods are recommended to confirm the potential use of Clitoria ternatea as potential source of antioxidant compound and maximize its use.

 

Keywords: 
Clitoria ternatea; Phenol; Flavonoid; Antioxidant property; DPPH
 
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