Isolation of IgG from chicken serum using a combination of coconut caprylic triglyceride and ammonium sulfate precipitation
Faculty of Animal Science, University of Mataram, Mataram - NTB, Indonesia.
Research Article
GSC Biological and Pharmaceutical Sciences, 2024, 27(02), 302–306.
Article DOI: 10.30574/gscbps.2024.27.2.0241
Publication history:
Received on 21 March 2024 revised on 25 May 2024; accepted on 28 May 2024
Abstract:
This study aimed to isolate IgG from chicken serum using a combination of coconut caprylic triglyceride and ammonium sulfate precipitation techniques. This study used serum from chickens that had previously been vaccinated repeatedly using a commercial rabies vaccine. Initially, the serum was treated with 2.5% coconut caprylic triglyceride, followed by serum IgG precipitation using 40% ammonium sulfate. The 10% SDS-PAGE analysis showed that a protein of about 190 kDa with a purity above 95% was successfully isolated. Whether the isolate protein is IgG and specific to rabies antigen still needs to be investigated further.
Keywords:
Antibody; Coconut caprylic triglyceride; Chicken serum; IgG; IgY
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Copyright © 2024 Author(s) retain the copyright of this article. This article is published under the terms of the Creative Commons Attribution Liscense 4.0
