In vitro propagation of Aloe vera Linn from shoot tip culture
Ethiopian Institute of Agricultural Research, Melkassa Agricultural Research Center, Ethiopia
Research Article
GSC Biological and Pharmaceutical Sciences, 2018, 04(02), 01–06
Article DOI: 10.30574/gscbps.2018.4.2.0052
Publication history:
Received on 20 June 2018; revised on 11 July 2018; accepted on 18 July 2018
Abstract:
Aloe vera Linn., plant belongs to Liliaceae family, is a plant originated in the warm, dry climates of Africa readily adaptable and grows worldwide. Slow propagation rate of axillary shoots and male sterility to use its seeds hinders its efficient propagation. Hence, the present study aimed to standardize optimum in vitro propagation conditions for it. Based on this purpose MS media supplemented with 0.6 mg/L BAP and 0.2 mg/L of IBA; 1.0 mg/L BAP; and devoid of hormone were selected as the optimum medium for Aloe vera establishment, multiplication and rooting respectively. The higher mean number of shoots, 2.90 and multiplication factor, 2.87 were registered on MS media with 0.2 mg/L IBA & 1.0 mg/L BAP respectively. During their rooting and acclimatization, the selected treatment yields 100% rooted plant and 92.59 survival percentage during acclimatization on 3:1 mixture of filter cake and sand after their fifth week. Application of this protocol helps to propagate thousands of Aloe vera plants from few initial mother plants in year period.
Keywords:
Aloe vera Linn; MS media; BAP; IBA; In vitro propagation
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Copyright © 2018 Author(s) retain the copyright of this article. This article is published under the terms of the Creative Commons Attribution Liscense 4.0.
