Sterility of cord blood units: Evaluation of the collection, preparation and conservation process with a view to establishing a bank of stem cells from cord blood at the Biological Resources Center of the Institut Pasteur de Côte d’Ivoire (2024)
1 Department of Biological Resources Center and Biobank, Institut Pasteur de Côte d’Ivoire, Côte d’Ivoire.
2 Technical and Technological Department, Molecular Genetics Platform, Institut Pasteur de Côte d’Ivoire, Côte d’Ivoire.
3 Technical and Technological Department, Cellular Biology Unit, Institut Pasteur de Côte d’Ivoire, Côte d’Ivoire.
4 Department on Parasitology-Mycology, Mycology Unit, Institut Pasteur de Côte d’Ivoire, Côte d’Ivoire.
5 Department of Bacteriology and Virology, Institut Pasteur de Côte d’Ivoire, Côte d’Ivoire.
Research Article
GSC Biological and Pharmaceutical Sciences, 2024, 29(01), 331–339.
Article DOI: 10.30574/gscbps.2024.29.1.0366
Publication history:
Received on 25 August 2024; revised on 25 October 2024; accepted on 28 October 2024
Abstract:
Introduction: Cord blood stem cells are used as an alternative to bone marrow and peripheral blood cell transplantation. For this purpose, cord blood banks have been established worldwide for the collection and cryopreservation of cord blood units. These banks must implement rigorous protocols to ensure the microbiological safety of grafts. The aim of this study is to evaluate the process of selecting mothers and collecting and processing these samples developed for this purpose.
Methodology: Pregnant women were enrolled in the study on the basis of selection criteria. Maternal blood and cord blood samples were collected by the in utero method. Serological analyses of maternal blood for the detection of vertically transmitted infections and microbiological examinations of cord blood units were carried out.
Results: 31 women out of 46 (68.4%) were enrolled. All had negative serology for the targeted pathogens. Similarly, microbiology revealed no microbial contamination of the cord blood units.
Conclusion: This work demonstrates that the procedures for selecting mothers and processing implementations are effective in ensuring the microbiological safety of cord blood units and could be suggested in the context of banking cord blood stem cells at the Institut Pasteur de Côte d’Ivoire.
Keywords:
Stem cells; Cord blood units; Cord blood stem cell bank; Microbiological safety; Institut Pasteur de Côte d’Ivoire
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Copyright © 2024 Author(s) retain the copyright of this article. This article is published under the terms of the Creative Commons Attribution Liscense 4.0
