Spray incorporation of Bacillus subtilis and Saccharomyces cerevisiae with different binding agents into granules for Oreochromis niloticus
1 Department of Biochemistry-Genetics, UFR Biological Sciences, Univ Peleforo Gon Coulibaly, BP 1328 Korhogo, Côte d'Ivoire.
2 Laboratory of Biology and Health, UFR Biosciences, Felix Houphouët Boigny Univ, Abidjan 01 BP V34, Abidjan, Côte d'Ivoire.
3 UMR MARBEC (MARine Biodiversity, Exploitation and Conservation), Univ Montpellier, CNRS, Ifremer, IRD, Sète, France.
4 UMR MARBEC (MARine Biodiversity, Exploitation and Conservation), Univ Montpellier, CNRS, Ifremer, IRD, Montpellier, France.
Research Article
GSC Biological and Pharmaceutical Sciences, 2025, 30(01), 223-232.
Article DOI: 10.30574/gscbps.2025.30.1.0015
Publication history:
Received on 09 December 2024; revised on 17 January 2025; accepted on 20 January 2025
Abstract:
In aquaculture, probiotics appear as one of the alternatives to the excessive use of antibiotics. They promote good health, growth and improve the quality of fish culture water. One route of administration is incorporation into foods. But the mode of incorporation influences their viability and density. The objective of this study is to evaluate the effectiveness of incorporation of Saccharomyces cerevisiae and Bacillus subtilis by spraying with three binding agents (sterile salt water 0.9 %, vegetable oil and egg white). After determining the type of interaction existing between the two probiotics, 3×109 CFU of each of them was sprayed onto 30 g of granules. Then, the viability of the incorporated probiotics was evaluated over 12 days and after 60 minutes of residence in water. The density of incorporated probiotics showed a significant difference with vegetable oil in S. cerevisiae for an average of 3.28×107 CFU/g. This density varies from 1.5×106 to 2.75×106 CFU/g with B. subtilis among all binders. For the viability of 12 days, we observe a progressive increase in microbial density with salt water as a binder in B. subtilis from 2.4×104 to 5.35×106 CFU/g and S. cerevisiae of 7.43×106 to 1.5×107 CFU/g. The contact test on water with B. subtilis shows a decrease in microbial density from 3.5×106 to 1.56×106 CFU/g, while with S. cerevisiae it favors an increase from 3.07×107 to 4, 56×108 CFU/g with all binding agents. Sterile salt water 0.9% is the appropriate binding agent for both probiotic strains.
Keywords:
Aquaculture; Bacillus subtilis; incorporation; probiotic; Saccharomyces cerevisiae
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Copyright © 2025 Author(s) retain the copyright of this article. This article is published under the terms of the Creative Commons Attribution Liscense 4.0
