The effect of Centella asiatica to the vascular endothelial growth factor and vascular endothelial growth factor receptor-2 on the rotenone induced zebrafish larvae (Danio rerio) stunting model
1 Master of Midwifery Program, Faculty of Medicine Universitas Brawijaya, Malang, Indonesia.
2 Institute of Health Science Sint Carolus Jakarta, Jakarta, Indonesia.
3 Department of Pharmacology, Faculty of Medicine, Universitas Brawijaya, Indonesia.
4 Departement of Biochemistry-Biomolecular Faculty of Medicine, Universitas Brawijaya, Indonesia.
5 Institute of Health Science William Booth Surabaya, Surabaya, Indonesia.
6 Midwifery Academy Poltekes Kemenkes Mataram, West Nusa Tenggara, Indonesia.
7 Midwifery Academy of Jember, Jember, East Java, Indonesia.
8 Midwifery academy of Berlian Nusantara, Magetan, Indonesia.
9 Midwifery Academy Dr. Soebandi, Jember, East Java, Indonesia.
10 University of Darul Ulum Jombang High Islamic Boarding School, Jombang, East Java, Indonesia.
Research Article
GSC Biological and Pharmaceutical Sciences, 2018, 05(02), 088–095.
Article DOI: 10.30574/gscbps.2018.5.2.0117
Publication history:
Received on 16 October 2018; revised on 26 October 2018; accepted on 01 November 2018
Abstract:
Stunting is a condition of obstructed body length growth in the first two year of life. Rotenone as a pesticide specimen which trigger stunting condition, inhibits the mitochondrial complex I causing an increased level of ROS that affects angiogenesis and vasculogenesis process that involve VEGF with VEGFR-2. Centella asiatica serves as antioxidant who inhibits ROS production and revitalize blood vessels. The purpose of this research is to discover the effect of Centella asiatica ethanolic extract to the enhancement of body length through the expression of the VEGF and VEGFR-2 on the rotenone-induced zebrafish larvae stunting model. The result in this study proved that [12.5 ppb] rotenone could induce stunting growth in 6 dpf (Days post fertilization) zebrafish larvae (Danio rerio). The administration of Centella asiatica ethanolic extract could improve the body length, along with the increasing of VEGF [2.5 mg/ml] and VEGFR-2 [5 mg/ml]. The correlation test shows a positive and strong relevance between the levels of Centella asiatica and the expression of VEGF and VEGFR-2. It can be concluded that Centella asiatica ethanolic extract could inhibit stunting and improved the body length through increasing the expression of VEGF and VEGFR-2 in stunting model zebrafish larvae.
Keywords:
VEGF; VEGFR-2; Stunting; Zebrafish; Centella asiatica
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Copyright © 2018 Author(s) retain the copyright of this article. This article is published under the terms of the Creative Commons Attribution Liscense 4.0
