Green synthesis and biochemical characterization of silver nanoparticles by using Euphorbia umbellata leaf extract and analysis of antimicrobial activity against plant pathogens

Sahu Ajay Kumar 1, Harichandan S.S Priyadarshini 2, Acharya Prangya Paramita 3, Samal Pinki 4, Gautam Sakshi 5, Barsha Rani Kar 6 and B Kumar Kiran 7, *

1 Department of Microbiology, Bangalore University, Bangalore, Karnataka.
2 Department of Biotechnology, Berhampur University, Odisha.
3 Department of Biotechnology, Sambalpur University, Odisha.
4 Department of Microbiology, Sambalpur University, Odisha.
5 Department of Biotechnology, Lovely Professional University, India.
6 Department of Biotechnology, Utkal University, Bhubaneswar. Odisha
7 Department of Biotechnology, Reva University, Bangalore 
 
Research Article
GSC Biological and Pharmaceutical Sciences, 2019, 08(02), 059–069.
Article DOI: 10.30574/gscbps.2019.8.2.0137
Publication history: 
Received on 25 July 2019; revised on 07 August 2019; accepted on 14 August 2019
 
Abstract: 
The science of nanotechnology and nanoparticles is the manipulation of matter on atomic and molecular weight, the nanotechnology and nanomaterial’s refer to the particular technological goal which is precise manipulation of atom and molecules for used the fabrication process of microbial products and it is now concern to as molecular nanotechnology, it also known as the nanotechnology is science of designing, making and application of nano-structure and nanomaterials also used investigation of relationship various properties of materials with their nanometer dimensions. The exploitation of various plant materials for the biosynthesis of silver nano particles is considered a green technology. Because it does not involves any harmful chemicals. Nanotechnology field is one of the most attractive researches. The field of nanotechnology is applied to bio materials. Nanoparticles are generally considered as particles with a size up to 100 nm, that have completely new or improved properties as compared to the bulk material that they are collected based on particular characteristics such as size, distribution and morphology. Different groups of unorganized parts of the plants have been utilizing for the green synthesis of silver nanoparticles, in the present work the fresh leaves of euphorbia umbellate have been used for the synthesis of silver nanoparticles. Synthesis of AgNPs employing either microorganisms or plant extracts has emerged as an alternative approach. Silver nanoparticles is embedded with antibacterial properties because of its unique properties is considered in medical science, the main aim of work is green synthesis of silver nanoparticles using Euphorbia Umbellate leaf extract and its antibacterial activity, after the collection of sample, identification and extraction of Euphorbia Umbellate was performed the production of silver nanoparticles.
Keywords: 
Silver nanoparticles; Biochemical characterization; Antibacterial activity; TEM and SEM analysis; UV spectroscopy; XRD analysis
 
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