In vitro propagation of Satureja thymbra L. (Lamiaceae): A valuable aromatic-medicinal native plant of the Mediterranean region
Laboratory of Protection and Evaluation of Native and Floricultural Species, Balkan Botanic Garden of Kroussia, Institute of Plant Breeding and Genetic Resources, Hellenic Agricultural Organization (HAO) – DEMETER, P.O. Box 60458, P.C. 570 01, Thermi, Thessaloniki, Greece.
Research Article
GSC Biological and Pharmaceutical Sciences, 2019, 09(02), 009-020.
Article DOI: 10.30574/gscbps.2019.9.2.0190
Publication history:
Received on 01 October 2019; revised on 24 October 2019; accepted on 26 October 2019
Abstract:
Satureja thymbra L. or savory is a highly threatened aromatic-medicinal endemic Mediterranean plant. Micropropagation can play a role in the protection of the natural ecosystem. The disinfection protocol involved the immersion of shoot-tip explants in 70% alcohol (1 min) and 3% NaOCl (15 min) giving 61.18% success. The MS medium supplemented with 30 g/l sucrose and 7 g/l Plant Agar was optimum for the initial establishment stage (10-day culture, 92.31% proliferation) and afterwards supplemented extra with 0.5 mg/l BA for multiple shoot induction (5 weeks) (100% proliferation, 5.5 shoots 14.24 mm long). In rooting stage, 1 mg/l IBA gave the highest rooting (58.62%), 0.5 mg/l NAA the maximum root number (12.13) and 0.1 mg/l IAA the largest root length (34.35 mm) (MS medium + 20 g/l sucrose + 6.5 g/l Plant Agar, 7 weeks) (Experiment No. 1). There was a progressive increase in rooting and shoot proliferation increasing the number of subcultures, being highest (100% rooting, 6 roots 70 mm long, 38.1% shoot multiplication, 2 shoots 30 mm long) in the 4th subculture/ 4.5 weeks each in WPM medium (Experiment No. 2). Therefore, best rooting results (50-100%) were accomplished in 3 different composition media: (1 and 2) WPM medium + 20 g/l sucrose + 6 g/l Plant Agar (1st and 4th subculture) and (3) MS medium + 30 g/l sucrose + 1 mg/l IBA + 7 g/l Plant Agar. The ex vitro survival of rooted microshoots after 4 weeks in the greenhouse mist (1 peat moss: 1 perlite) was 81.82%.
Keywords:
Ex situ conservation; Mediterranean endemic; Micropropagation; Plant growth regulators; Satureja thymbra; Tissue culture media
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