Phytochemical studies of Cissus populnea in Benue State, Nigeria
DOI:
https://doi.org/10.30574/gscbps.2018.2.1.0022Keywords:
Phytochemical, Ethyl acetate, GC-MS, Cissus populneaAbstract
Phytochemical studies of Cissus populnea in Benue State Nigeria was carried out to determine the presence of different chemicals in the stem, based on locality. The state was stratified into three zones using the already existing political grouping. From each of the zones samples of plants were collected with a minimum of 100 m apart. This is to avoid collection from the same siblings. The collected samples were air dried and pounded to paste. The obtained paste was soaked in hexane (150 ml), ethyl acetate (150 ml) and methanol (150 ml) for 48 h each. The mixtures were agitated every 4 h and then filtered in glass jars (1 L) representing the locations for each zone. The filtrates were left open to evaporate to dryness. After that, extracts were transferred into vials. The ethyl acetate extracts were coded for identification and sent for GC-MS analysis. The results obtained confirmed the presence of different chemicals in the stem of the plants and there was variation in the type of chemical obtained based on location.
Metrics
References
Ajibesin KK. (2011). Dacryodes edulis (G. Don) HJ. Lam: A review on its medicinal, phytochemical and economical properties. Research Journal of Medicinal Plant, 5(1), 32–41.
Akinpelu DA and Onakoya, Z. T. M. (2006). Antimicrobial activities of medicinal plants used in folklore remedies in south-western Nigeria. African Journal of Biotechnology, 7(5), 1078 –1081.
Mojab F, Kamalinejad M, Ghaderi N and Vanidipour HR. (2003). Phytochemicals screening of some species of Iranian plants. Iranian Journal of Pharmaceutical Research, 2(2), 77–82.
Parekh J and Chanda S. (2007). Antibacterial and phytochemical studies on twelve species of Indian medicinal plants. African Journal of Biomedical Resource, 10, 175–181.
Parekh J and Chanda S. (2008). Phytochemicals screening of some plants from western region of India. Plant Archives, 8, 657–662.
Eleazu CO, Eleazu KC, Awa E and Chukwuma SC. (2012). Comparative study of the phytochemical Composition of the leaves of five Nigerian medicinal plants. Journal of Biotechnology and Pharmaceutical Research, 3(2), 42–46.
Rowley AG. (2001). Evaluating Uncertainty for Laboratories. A Practical Handbook.
Sahil K, Prashant B, Akanksha M, Premjeet S and Devashish R. (2011). GC-MS: Applications. International Journal of Pharmacology and Biological Archives, 2, 1544–1560.
Ojekale AB, Lawal OA, Lasisi AK and Adeleke TI. (2006). Phytochemistry and spermatogenic potentials of aqueous extract of Cissus populnea (Guill and perr) stem bark. Science World, 6, 2140–2146.
Jenke DR. (1996). Chromatographic method validation: a review of current practices and procedures. II Guidelines for primary validation parameters. Journal of Liquid Chromatography and Related Technology, 19(5), 737– 757.
Varsha J, Vaibhav K and Poonam P. (2014). GC-MS analysis of bioactive compounds in methanolic extract of Holigarna grahamii (Wight) Kurz. International Journal of Herbal Medicine, 2(4), 35–39.
Srinivasan K, Sivasubramanian S and Kumaravel S. (2013). Phytochemical profiling and GC-MS study of Adhatoda vasica leaves. International Journal of Pharmacology and Biological Archives, 5(1), 714–720.
Dukes J. (2013). Phytochemical and ethnobotanical databases. www.arsgov/ cgi-bin/duke/.
Onojah PK, Odin EM and Adegbe A. (2015). Comparative analysis of the nutritional contents of the fresh stem exudates and the dried pulp of the root of Cissus populnea plant found in Anyigba, Kogi State, Nigeria. Journal of Science, 4(3), 386 –389.
Moody JO, Ojo OO, Omotade OO, Adeyemo AA, Olumese PE and Ogundipe OO. (2003). Anti-sickling potential of a Nigerian herbal formula (ajawaron HF) and the major plant components (Cissus populnea L. CPK). Phytotherapy Research. 17(10), 1173 – 1176.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2018 Mark Uleh, Anyam Vershima, Sanni Ojogbayi
![Creative Commons License](http://i.creativecommons.org/l/by-nc-sa/4.0/88x31.png)
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.