Phytochemical studies of Cissus populnea in Benue State, Nigeria

Mark Uleh; Anyam Vershima; Sanni Ojogbayi

doi:10.30574/gscbps.2018.2.1.0022

Authors

Mark Uleh Department of Forest Production and Products, University of Agriculture, Makurdi, Nigeria.
Anyam Vershima Center for Agrochemical Technology, Department of Chemistry, Federal University of Agriculture Makurdi, Nigeria.
Sanni Ojogbayi Department of Forest Production and Products, University of Agriculture, Makurdi, Nigeria.

DOI:

https://doi.org/10.30574/gscbps.2018.2.1.0022

Keywords:

Phytochemical, Ethyl acetate, GC-MS, Cissus populnea

Abstract

Phytochemical studies of Cissus populnea in Benue State Nigeria was carried out to determine the presence of different chemicals in the stem, based on locality. The state was stratified into three zones using the already existing political grouping. From each of the zones samples of plants were collected with a minimum of 100 m apart. This is to avoid collection from the same siblings. The collected samples were air dried and pounded to paste. The obtained paste was soaked in hexane (150 ml), ethyl acetate (150 ml) and methanol (150 ml) for 48 h each. The mixtures were agitated every 4 h and then filtered in glass jars (1 L) representing the locations for each zone. The filtrates were left open to evaporate to dryness. After that, extracts were transferred into vials. The ethyl acetate extracts were coded for identification and sent for GC-MS analysis. The results obtained confirmed the presence of different chemicals in the stem of the plants and there was variation in the type of chemical obtained based on location.

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