Hydrocarbonoclastic potentials of Enterobacteriaceae isolated from the crude oil polluted Iko river estuary and freshwater ecosystem of the Niger Delta Region of Nigeria

Abigail Johnny Nkanang; Sylvester Peter Antai; Atim David Asitok; Maurice Ekpenyong

doi:10.30574/gscbps.2018.2.1.0058

Authors

Abigail Johnny Nkanang Department of Microbiology, University of Calabar, Cross River State, Nigeria
Sylvester Peter Antai Department of Microbiology, University of Calabar, Cross River State, Nigeria
Atim David Asitok Department of Microbiology, University of Calabar, Cross River State, Nigeria
Maurice Ekpenyong Department of Microbiology, University of Calabar, Cross River State, Nigeria

DOI:

https://doi.org/10.30574/gscbps.2018.2.1.0058

Keywords:

Crude oil, Iko river, Hydrocarbonoclastic, Enterobacteriaceae, Bioaugmentation, Bioremediation

Abstract

Hydrocarbonoclastic potentials of Enterobacteriaceae isolated from the crude oil polluted Iko river estuary and freshwater ecosystem of the Niger Delta was investigated. The isolation of crude oil utilizing bacterial isolates in water sample was carried out by surface spreading technique using diluents prepared with quarter strength Ringers solution and cultured on oil agar medium (OAM). Of the 32 potential hydrocarbon utilizing bacteria from crude oil impacted aquatic ecosystem only eight isolates identified as; Citrobacter amalonaticus–Y1 (FSW); Proteus mirabilis strain–I(FSW), Pseudomonas fluorescens–N (FSE); Citrobacter farmeri–Y12 (FSE), Citrobacter amalonaticus strain–Y2 (ESWS1), Enterobacter sp.–Y8 (ESWS3), Proteus mirabilis strain–K (ESWS1), Proteus penneri strain–O(ESES3) were found to demonstrate strong hydrocarbonoclastic potentials with variable levels of low pH and increase optical density and free carbon iv oxide production. The study revealed that Citrobacter amalonaticus strain–Y2 (ESWS1) of the family Enterobacteriaceae is a good candidate for bioaugmentation technique of bioremediation.

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References

Muncaster SP, Jacobson G, Taiarui M, King S and Bird S. (2016). Effects of MV Rena heavy fuel oil and dispersed oil on yellowtail kingfish early life stages. New Zealand Journal of Marine and Freshwater Research, 50(1), 131-143.

Adams GA, Fufeyin PT, Okoro SE and Ehinomen I. (2015). Bioremediation, biostimulation and bioaugmention: A review. International Journal of Environmental Bioremediation and Biodegradation, 3(1), 28-39.

Olukunle OF. (2013). Characterization of indigenous microorganisms associated with crude oil-polluted soils and water using traditional techniques. Microbiology Journal, 3, 1-11.

Chikere CB, Okpokwasili GC and Chikere BO. (2011). Monitoring of microbial hydrocarbon remediation in the soil. Biotechnology, 3(1), 117–138.

Javaid MK, Ashiq M and Tahir M. (2016). Potential of biological agents in decontamination of agricultural soil. Scientifica, 1-9.

Chu D and Barnes DJ. (2016). The Lag-phase during diauxic growth is a trade-off between fast adaptation and high growth rate. Scientific reports, 6, 25191.

Oyedeji AA. (2016). Impacts of selected leguminous tree species and kaolinite pre-amendment on oil-contaminated soil for bioremediation in the oil-bearing region of Nigeria. Ph. D. thesis, University of Wolverhampton, UK.

Etuk CU, John RC, Ekong UE and Akpan MM. (2012). Growth study and hydrocarbonoclastic potential of microorganisms isolated from aviation fuel spills site in Ibeno. Nigeria Bulletin of Environmental Contamination and Toxicology, 89, 727-732.

Chikere CB, Okpokwasili GC and Ichiakor O. (2009). Characterization of hydrocarbon utilizing bacteria in tropical marine sediments. African Journal of Biotechnology, 8 (11), 2541-2544.

Lee K, Boudreau M, Bugden J, Burridge L, Cobanli SE, Courtenay S, Grenon S, Hollebone B, Kepkay P and Li Z. (2011). State of knowledge review of fate and effect of oil in the arctic marine environment. A report prepared for the National Energy Board of Canada.

John RC, Itah AY, Essien JP and Ikpe DI. (2011). Fate of nitrogen-fixing bacteria in crude oil contaminated wetland ultisol. Bulletin Environmental Contamination and Toxicology, 87, 343–353

Reghuvaran A, Jacob K and Ravindranath, A. (2012). Isolation and characterization of nitrogen fixing bacteria from raw Coir Pith. African Journal of Biotechnology, 11(27), 7063-7071.

Akan OD, Etok CA and Adegoke AA. (2013). Bioremediation of crude oil contaminated soil using wood ash. Journal of Pure and Applied Microbiology, 7(3), 1749-1756.

Croquer A, Bone D, Bastidas C, Ramos R and García E. (2016). Monitoring coastal pollution associated with the largest oil refinery complex of Venezuela. PeerJ 4, e2171.

Abdulsalam S, Adefila SS, Bugaje IM and Ibrahim S. (2013). Bioremediation of soil contaminated with used motor oil in a closed system. Bioremediation and Biodegradation, 3, 100-172.

Jain PK, Gupta VK, Gaur RK, Lowry M, Jaroli DP and Chaukan UK. (2011). Bioremediation of petroleum oil contaminated soil and water. Research Journal of Environment Toxicology, 5(1), 1 – 26.

Udotong IR, Eduok SI, Essien JP and Ita BN. (2008). Density of hydrocarbonoclastic bacteria and polycyclic aromatic hydrocarbon accumulation in Iko river mangrove ecosystem, Nigeria. International Journal of Environmental, Chemical, Ecological, Geological and Geophysical Engineering, 2(8), 121-127.

American Public Health Association (APHA). (2005). Standard methods for the examination of water and wastewater. 21st ed. American Public Health Association, Washington DC, 1220.

Zajic JE and Supplisson B. (1972). Emulsification and degradation of “Bunker C” fuel oil by microorganisms. Biotechnology and Bioengineering, 14(3), 331-343.

Asitok,A. D.,Antai, S.P. and Ekpeyong, M. G. (2017).Water soluble fraction of crude oïl uncouples protease biosynthesis and activity in hydrocarbonoclastic bacteria ; implications for natural attenuation. International Journal of Sciences, 6(7), 5-21.

Esin AA and Antai SP. (2002). Biodegradability of the major components of Bonny light crude oil by Bacillus subtilis. Global Journal of pure and Applied Science, 8 (2), 215-222.

Vanishree M, Thatheyus AJ and Ramya D. (2014). Biodegradation of petrol using Aspergillus sp. Annual Research and Review in Biology, 4(6), 914 – 923.

Itah AY and Essien JP. (2005). Growth profile and hydrocarbonoclastic potential of microorganisms isolated from tarballs in the Bight of Bonny, Nigeria. World Journal of Microbiology and Biotechnology, 21(6), 1317-1322.

Gomathy and Senthilkumar R. (2012). Distribution of hydrocarbanoclastic bacteria in Cuddalore coastal water and their emulsification potential on crude oil. International Journal of Research in Biological Sciences, 2(3), 107-112.

Irshaid FI and Jacob JH. (2015). Screening and characterization of aerobic xylene-degrading bacteria from gasoline contaminated soil sites around gas stations in Northern Jordan. Journal of Biological Sciences, 15(4), 167-176.

Obst MA, Krug H, Luftmann A and Steinbuchel K. (2005). Degradation of cyanophycin by Sedimentibacter hongkongensis strain KI and Citrobacter amalonaticus strain G isolated from an anaerobic bacterial consortium. Applied Environmental Microbiology, 71, 3642-3652.

Abdulsalam S, Bugaje IM, Adefila SS and Ibrahim S. (2011). Comparison of biostimulation and bioaugmentation for remediation of soil contaminated with spent motor oil. International Journal of Environmental Science and Technology, 8(1), 187-194.