Isolation and antibiotic susceptibility test of bacteria from healthcare workers and hospital environment
DOI:
https://doi.org/10.30574/gscbps.2018.4.1.0047Keywords:
Antibiotic susceptibility, Bacillus spp, Staphylococcus spp, Streptococcus sppAbstract
Hospital-acquired infections are one of the most significant adverse events for healthcare institutions increasing morbidity and mortality. The hospital environment is a potential source of infection especially with pathogenic bacteria. The study was aimed at identifying some possible sources of pyogenic bacterial infections in a Private hospital and determining the antibiotic susceptibility. Bacteriological analysis of swabs from hands of health care staff, the floors in the wards and clinics, beds and examination couches in the clinics and wards was carried out. Organisms were identified by Gram stain, catalase and coagulase test oxidase test. Bacillus spp and Streptococcus spp were isolated from the floor, bed and air. Staphylococcus spp was isolated from the hands of health workers. The organisms isolated were resistant to cloxacillin, amoxicillin, and streptomycin but susceptible to ofloxacin, gentamicin and co-trimoxazole. The findings in this study reveal that the hospital environment and the hands of healthcare workers can serve as a reservoir for antibiotic resistant microorganisms.
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References
WHO. (2016). The burden of health care-associated infection worldwide Available from: http://www.who.int/gpsc/country_work/burden_hcai/en/
Nejad SB, Benedetta A, Shamsuzzoha BS, Benjamin E and Didier P. (2011) Health-care-associated infection in Africa: a systematic review. Bulletin of the World Health Organization 89, 757-765.
Tacconelli E, Cataldo MA, Dancer SJ, De Angelis G, Falcone M, Frank U, Kahlmeter G, Dan A and Petrosillo N. (2014). ESCMID guidelines for the management of the infection control measures to reduce transmission of multidrug resistant Gram-negative bacteria in hospitalized patients. Clinical Microbiology and Infection, 20 (1), 1 – 55.
Ananthan G, Sivaperumal P and Hussain SM. (2011). Antibacterial potential of marine ascidian Phallusia Arabica against isolated urinary tract infections bacterial pathogens. Asian Journal of Animal Sciences, 5 (3), 208-212.
Elizabeth NM, Clement M and Uwem OE. (2016). Prevalence and antimicrobial susceptibility profile of bacteria isolated from the environment of two tertiary hospitals in Calabar Metropolis, Nigeria. Journal of Advances in Medical and Pharmaceutical Sciences, 10(4), Article no.JAMPS.29316.
Kollef MH and Fraser VJ. (2001). Antibiotic resistance in intensive care unit setting. Annals of Internal Medicine, 134, 298-314.
Shankar PR, Partha P, Dubey AK, Mishra P and Deshpande VY. (2005). Intensive care unit drug utilization in a teaching hospital in Nepal. Kathmandu University Medical Journal, 3, 130-137.
Clinical Laboratory Standards Institute. (2011). Performance standards for antimicrobial susceptibility testing. Twenty-first informational supplement, CLSI document M100-S21. PA, USA, Wayne.
Ige OK, Adesanmi AA and Asuzu MC. (2011). Hospital-acquired infections in a Nigerian tertiary health facility: An audit of surveillance reports. Nigerian Medical Journal, 52, 239-43.
Ikeh EI and Erdoo SI. (2011) Nigerian flora of formites in a Nigerian multidisciplinary intensive care unit. Laboratory Medicine, 42(7), 411-413.
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