Assessment of steviol on pro-inflammatory cytokines release in human CD14+ cells

Jatuporn Rattanasrisomporn; Waraporn Boonkaewwan; Autchara Kayan; Chaiwat Boonkaewwan

doi:10.30574/gscbps.2018.4.2.0048

Authors

Jatuporn Rattanasrisomporn Department of Companion Animal Clinical Science, Faculty of Veterinary Medicine, Kasetsart University, Bangkok, Thailand.
Waraporn Boonkaewwan Department of Preventive and Social Medicine, Faculty of Medicine, Srinakharinwirot University, Nakhonnayok, Thailand.
Autchara Kayan Department of Animal Science, Faculty of Agriculture, Kasetsart University, Bangkok, Thailand.
Chaiwat Boonkaewwan Department of Animal Science, Faculty of Agriculture, Kasetsart University, Bangkok, Thailand.

DOI:

https://doi.org/10.30574/gscbps.2018.4.2.0048

Keywords:

Steviol, TNF-α, IL-1β, IL-6, CD14 cell

Abstract

Steviol is a major metabolite of stevioside which is a natural noncaloric sweetener. The aim of this present study was to investigate an inhibitory activity of steviol on pro-inflammatory cytokines release from lipopolysaccharide (LPS)-stimulated human CD14⁺ cells. CD14⁺ cells were incubated with different concentrations of steviol in the absence or presence of LPS. TNF-α, IL-1β and IL-6 level were determined by using human enzyme-linked immunosorbent assay (ELISA) kits. Steviol at the concentration of 1-100 µM significantly inhibit (P<0.05) the release of TNF-α (966.0+66.0, 906.3+36.7 and 659.3+52.3 vs 1190.3+75.5 pg/ml) and IL-1β (2834.0+67.2, 2440.0+70.5 and 2181.3+143.3 vs 3226.7+106.6 pg/ml) in a dose dependent manner when compared to LPS-treated CD14⁺ cells. While the inhibition of IL-6 (P<0.05) was found at 10 µM and 100 µM (2171.7+44.3 and 1902.3+58.0 vs 2580.3+105.2 pg/ml). In conclusion, steviol, a major metabolite of stevioside, possess an anti-inflammatory activity in human CD14⁺ cell.

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References

Hanson JR and Oliveira BH. (1993). Stevioside and related sweet diterpenoid glycosides. National Product Report, 10, 301-309.

Geuns JM, Augustijins P, Mols R, Buyse JG and Driessen B. (2003) Metabolism of stevioside in pigs and intestinal absorption characteristics of stevioside, rebaudioside A and steviol. Food and Chemical Toxicology, 41(11), 1599-1607.

Koyama E, Sakai N, Ohori Y, Kitazawa K, Izawa O, Kakekawa K, Fujino A and Ui M. (2003). Absorption and metabolism of glycosidic sweeteners of stevia mixture and their aglycone, steviol, in rats and humans. Food and Chemical Toxicology, 41(3), 875-883.

Moon PD, Jeong HJ, Um JY, Kim HM and Hong SH. (2007). LPS-induced inflammatory cytokine production was inhibited by Hyungbang Jihwang Tang through blockade of NF-kappaB in peripheral blood mononuclear cells. International Journal of Neuroscience, 117(9), 1315-1329.

Bas E, Recio MC, Abdallah M, Manez S, Giner RM, Cerda-Nicolas M and Rios JL. (2007). Inhibition of the pro-inflammatory mediator production and anti-inflammatory effect of the iridoid scrovalentinoside. Journal of Ethnopharmacology, 110, 419–427.

Ogawa T, Nozaki M and Mitsui M. (1980). Total synthesis of stevioside. Tetrahedron, 36, 2641-8.

Boyum A. (1968). Isolation of mononuclear cells and granulocytes from human blood. Scandinavian Journal of Clinical and Laboratory Investigation, 21, 77-89.

Mosmann T. (1983). Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. Journal of Immunological Methods, 65(1-2), 55-63.

Gorduysus M, Avcu N, Gorduysus O, Pekel A, Baran Y, Avcu F and Ural AU. (2007). Cytotoxic effects of four different endodontic materials in humam periodontal ligament fibroblasts. Journal of Endodontics, 33(12), 1451-1454.

Chaiwat B, Mei A, Chaivat T and Mrinalini CR. (2008). Specific immunomodulatory and secretory activities of stevioside and steviol in intestinal cells. Journal of Agricultural and Food Chemistry, 56 (10), 3777–3784.

Buamann H and Gauldie J. (1994). The acute phase response. Immunology Today, 15, 74-89.

Morikawa K, Watabe H, Araake M and Morikawa S. (1996). Modulatory effect of antibiotics on cytokine production by human monocytes in vitro. Antimicrobial Agent and Chemotherapy, 4, 1366-1370.

Glauser MP. (1996). The inflammatory cytokines: new developments in the pathophysiology and treatment of septic shock. Drugs; 52(2), 9-17.

Jatuporn N, Narissara L, Autchara K and Chaiwat B. (2017). In vitro and in vivo assessment of inhibitory effect of stevioside on pro-inflammatory cytokines. Avicenna Journal of Phytomedicine, Avicenna Journal of Phytomedicine, 7(2), 101-106.