Phytochemical analysis and free radical scavenging activity of Isoberlinia doka leaves
DOI:
https://doi.org/10.30574/gscbps.2018.4.1.0049Keywords:
Isoberlinia doka, Free radical scavenging, Phytochemical analysis, 1, 1-diphenyl-2-picrylhydrazyl radical (DPPH) assayAbstract
Isoberlinia doka is dominant species in the savannah of West Africa belonging to the family Fabaceae. It is used in traditional medicine for treatment of muscular – skeletal system disorders, Jaundice scorpion bites, Convulsion, diabetes, ulcer, wounds and cough. The objectives of the study are to determine the pharmacognostic and antioxidant activity of Isoberlinia doka leaves. Three extracts of Isoberlinia doka leaves were obtained by sequential maceration using n-hexane, ethyl acetate and methanol The extracts were screened qualitatively for the presence of saponins, carbohydrates, tannins, flavonoids, cardiac glycosides, anthraquinones and alkaloids using standard procedures. Thin layer chromatography was carried out to determine the separation profile of various extracts. Antioxidant activity of the methanol leaves extract of Isoberlinia doka was determined using 1, 1-diphenyl-2-picrylhydrazyl radical (DPPH) Assay. The phytochemical screening of the methanol extract revealed the presence of alkaloids, flavonoids, tannins, cardiac glycosides and saponins. The methanol leaves extract of Isoberlinia doka demonstrated strong radical scavenging activity and reducing power ability with concentration dependent responses. The results of this study suggest that the plant can serve as a good source of antioxidant which can aid in the management of diseases associated with oxidative stress.
Metrics
References
Nithya N and Balakrishnan KP. (2011). Evaluation of some medicinal plants with antioxidant activity. International Journal of Pharmtech Research, 3(1), 381-385.
Schieber M and Chandel NS. (2014). ROS function in redox signaling and oxidative stress. Current biology, 24(10), 453-462.
Halliwell B and Gutteridge JMC. (1990). Role of free-radicals and catalitic metal ions in human disease: an overview. Methods Enzymology, 186, 1- 85.
Lai LS, Chou ST and Chao WW. (2001). Studies on the antioxidative activities of Hsiantsao (Mesona procumbens. Hems l.) leaf gum. Journal of Agricultural and Food Chemistry, 49, 963-968.
Mohamed M, Wadah JAO, Shereen OA, Amna A, Mahgoub SE. (2017). In vitro antioxidant activity of a flavonoid compound isolated from methanolic extract of Helianthus annuus leaves (Asteraceae). Der Pharmacia Letter, 9 (6), 28-34.
Kenneth AK. (1968). A survey of vegetation in Zaria. Vegetation, 15(4), 244-268.
Abdulkadir IE, Aliyu AB, Ibrahim MA, Audu SBD and Oyewale AO. (2011). Antioxidant activity and mineral elements profiles of Isoberlinia doka leaves from Nigeria. Australian Journal of Basic and Applied Sciences, 5(12), 2507-2512.
Kokate CK, Purohit AP and Gokhale SB. (1995). Pharmacognosy, 3rd edition, Nirali Prakashan, Pune.
Harborne JB. (1973). Phytochemical methods, A guide to modern technique of plant analysis, Chapman and Hill, London, 279.
Sofowora, A. (1984). Research on medicinal plants and traditional medicine in Africa. Journal of Alternative Complementary Medicine, 2, 365-372.
Mensor LL, Meneze FS, Leitao GG, Reis AS, Dos santor JC, Coube CS and Leitao SG. (2001). Screening of brazilian plant extracts for antioxidant activity by the use of DPPH free radical method. Phytotherapy research, 15, 127-130.
Akinmoladun AC, Ibukun EO, Afor E, Akinrinlola BL, Onibon TR, Akinboboye AO, Obuotor EM and Farombi EO. (2007). Chemical constituents and antioxidant activity of Alstonia boonei. African Journal of Biotechnology, 6(10), 1197-1201.
Shahidi F and Wanasundara PKJ. (1992). Phenolic antioxidants. Critical Review and Food Science Nutrition, 32, 67-103.
Kahkonen MP, Hopia AI, Vuorela HJ, Rauha JP, Pihlaja K, Kujala TS and Heinonen M. (1999). Antioxidant activity of plant extracts containing phenolic compounds. Journal of Agricultural and Food Chemistry, 47, 3954-3962.
Proestos C, Boziaris, I. S., Nychas, GJE and Komaitis M. (2006). Analysis of flavonoids and phenolic acids in Greek aromatic plants: investigation of their antioxidant capacity and antimicrobial activity. Food Chemistry, 95, 664-67.
Abdou MOA, Ambaliou S and Latifou L. (2015). Antioxidant activity and total phenolic, flavonoid and flavonol contents of the bark extracts of Acacia ataxacantha. Journal of Pharmacognosy and Phytochemistry, 4(2), 172-178.
Motalleb G, Hanachi P, Kua SH, Fauziah O and Asmah R. (2005). Evaluation of phenolic content and total antioxidant activity in Berberis vulgaris fruit extract. Journal of Biological Science, 5, 648-653.
Downloads
Published
How to Cite
Issue
Section
License
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.