Impact of polycyclic aromatic hydrocarbon effluent on the enzyme activity in blood serum of freshwater food fish Cirrhinus mrigala
DOI:
https://doi.org/10.30574/gscbps.2018.4.3.0066Keywords:
Polycyclic aromatic hydrocarbon, Cirrhinus mrigala, SGOT, SGPTAbstract
The purpose of this study was to estimate the acute toxicity of polycyclic aromatic hydrocarbon (PAH) effluent on Cirrhinus mrigala and to evaluate the lethal levels. The 120 hrs median lethal concentration of polycyclic aromatic hydrocarbon (PAH) effluent were found to be 20ppt for Cirrhinus mrigala. Further experiments were proceeded with sub lethal concentration of (1/10th conc. of LC50) polycyclic aromatic hydrocarbon (PAH) effluent which were evaluated from the LC50 value. After treatment the fishes were reared in ideal condition, then sacrificed dissected at different predetermined interval during the accumulation period, (i.e.) 1st day to 20th day, during the depuration period from 1st day to 15th day for Cirrhinus mrigala in polycyclic aromatic hydrocarbon (PAH) effluent treatment for assay studies. The enzyme activity studies carried out under sub lethal (1/10th conc. of LC50) in Blood Serum. The present study indicates that oil effluent induced alterations in the enzymatic activities of the freshwater food fish both at acute and sub-lethal concentrations. These alterations can be considered as a tool for biomonitoring of pharmaceutical drug substances in the aquatic environment. The present work also indicates that oil effluent causes considerable alterations in enzymes activities and is likely to induce tissue damage in Cirrhinus mrigala. Therefore, this effluent should be handling with care and prevent its entrance into aquatic environment.
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References
Jhingram, VG. (1991). Fish and Fisheries of India, 3rd edition Hindustan Publishing Corp. (India) New Delhi 727.
Smolowitz, RM, Hahn ME and Stegemann JJ. (1991). Immunochemical localization of cytochrome P450 1A induced by 3,3,4,4, tetrachlorobiphenyl and 2, 3, 7, 8 tertrachlorobenzofuran in liver and extra hepatic tissues of the teleost, Stenotomuschrysops. Drug Metabolism & Disposition, 19, 113-123.
Fleeger, JW, Carman KR and Nisbet RM. (2003). Indirect effects of contaminants in aquatic ecosystems. Science of the Total Environment, 317, 207-233.
Vutukuru, SS, Chintada S, Madhavi KR, Rao JV and Anjaneyulu Y. (2005). Acute effects of copper on superoxide dismutase, catalase and lipid peroxidation in the freshwater teleost fish, Esomusdanricus. Fish Physiology and Biochemistry, 32(3), 221-229.
Ebrahimpour, M and Mushrifah I. (2010). Seasonal Variation of Cadmium, Copper and Lead Concentrations in Fish from a Freshwater Lake. Biological Trace Element Research, 1-3, 191-201.
Lonsdale, DJ, Cerrato RM, Holland R, Mass A, Holt L and Schaffner RA. (2009). Influence of suspension-feeding bivalves on the pelagic food webs of shallow, coastal embayments. Aquatic Biology, 6, 263-279.
Dutta HM and Dalal R. (2008). The effect of endosulfan on the ovary of bluegill sunfish: a histopathological study (Lepomismacrochirussp). International Journal of Environmental Research, 2, 215-224.
De AK. (1996). Environmental Chemistry, 3rd edition, New Age International Pvt. Ltd. New Delhi.
Ambrose T, Vincent S and Cyril L. (1994). Susceptibility of the freshwater fish Gambusia affinis (Baird and Girard), Sarotherodon mossambicus (Peters) and Cirrhinus mrigala (Ham) to Zinc toxicity. Indian Journal of Environment and Toxicology, 4, 29-31.
Saravanan, TS, Aneez Mohamed M and Harikrishnan R. (2000). Studies on the chronic effects of Endosulfan on blood and liver of Oreochromis mossambicus. J Ecol Res Biocon, 1, 24-27.
Wang CS, Chang Ting-Tsung, Yao, Weijen, Wang, Shan-Tair, Chou and Pesus. (2012). Impact of increasing alanine amino transferase levels within normal range on incident diabetes. Journal of the Formosan Medical Association, 111, 201.
Dong X, Zhu L, Wang J, Xie H, Hou X and Jia W. (2009). Effects of atrazine on cytochrome P450 enzymes of zebrafish (Daniorerio). Chemosphere, 77, 404.
Ghorpade, N., Mehta, V., Khare, M., Sinkar, P, Krishnan, S and Rao, C. V. (2002). Toxicity study of diethyl phthalate on freshwater fish Cirrhina mrigala. Ecotoxicol. Environ. Saf, 53, 255–258.
Mathan R. (2006). Studies on the impact of heavy metal cadmium on certain enzymes in a freshwater teleost fish, Cyprinus carpio. Toxicology Letters, 164, 157-161.
Antonella, V and Landriscina C. (1999). Changes in liver enzyme activity in the teleost Sparus aurata in responses to cadmium intoxication. Ecotoxicology and Environmental Safety, 43, 111-116.
Zikic RV, Stajn AS, Pavlovic SZ, Ognjanovic BI and Saicic ZS. (2001). Activities of superoxide dismutase and catalase in erythrocytes and plasma transaminases of goldfish (Carassius auratus gibelio Bloch.) exposed to cadmium. Physiological Research, 50, 105-111.
De la Torre FR, Salibian A and Ferrari L. (2000). Biomarkers assessment in juvenile Cyprinus carpio exposed to waterborne cadmium. Environmental Pollution, 109, 277-282.
Velmurugan, B, Ambrose T and Selvanayagam M. (2006). Genotoxic evaluation of lambda cyhalothrin in Mystus gulio. Journal of Environmental Biology, 27, 247-250.
Sadhu, KA, Chowdhury and Mukhopadhyay. (1985). Relationship between serum enzymes, histological features and enzymes, histological features and enzymes in hepato pancreas after sub lethal exposure to malathion and phophamidon in the murrel Channa striatus (B.L.). International Journal of Environmental Studies, 24, 35-41.
Winkaler, EU, Santos TRM, Machado-Neto JG and Martinez CBR. (2007). Acute lethal and sublethal effects of neem leaf extracts on the neotropical freshwater fish Prochilodus lineatus. Comparative Biochemistry and Physiology, 145, 236-244.
Bhatnagar, MC and Tyagi M. (1995). Pyrethroid induced alternations in transaminases in liver and muscle of Clarias batrachus (Linn). Proceedings of the Academy of Environmental Biology, 4(2), 251-253.
Saqib, TA, Naqvi SN, Siddiqui PA and Azmi MA. (2005). Detection of pesticide residues in muscles, liver and fat of 3 species of Labeo rohita found in Kalri and Haleji lakes. Journal of Environmental Biology, 26, 433-438.
Sastry, KV and Sharma K. (1980). Mercury induced haematological and biochemical anomalies in Ophiocephalus, Channa Punctatus. Journal of Environmental Biology, 18, 291.
Hilmy AH, Shabano MB and Daabes AY. (1985). Bioaccumulation of cadmium: Toxicity in Mugilcephalu. Comparative Biochemistry and Physiology, 81, 139-144.
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