Free radical scavenging activity of two edible vegetables from the Niger delta region of Nigeria
DOI:
https://doi.org/10.30574/gscbps.2018.5.2.0092Keywords:
Gongronema latifolium, Lasianthera africana, Free radical, PhytochemicalAbstract
Oxidative stress and diseases that have their aetiology and pathophysiology in reactive oxygen species can be prevented by medicinal plants and herbs. We undertook in this study, to investigate the free radical scavenging activity of dichloromethane (DCM) and ethanol (ETOH) extracts of Lasianthera africana and Gongronema latifolium in vitro using 2, 2, diphenyl-1-picrylhydrazine (DPPH). The leaves of both plants were obtained locally, washed, dried and pulverized using mechanical grinder. The powdered materials were defatted using n-hexane and subsequently extracted with DCM and thereafter with ETOH. The DPPH scavenging potentials of the extracts were then evaluated. The percentage inhibition of the DCM and ETOH extracts were; 46.2 and 80% respectively for L. africana and 37.2 and 82.6 respectively for G. latifolium. The standard substance (quercetin) produced 98.4 per cent inhibition at 1 mg/mL. The IC50 for the L. africana were; 2.017 and 3.256 mg/mL; DCM and ETOH extracts respectively. While that of G. latifolium were 1.495 and 1.116 mg/mL respectively for DCM and ETOH extracts. The standard substance produced IC50 of 0.55 mg/mL. The DPPH inhibitory activity was found to be prominent with the ETOH extracts of both plants. The result from this study validates the use of the leaves of both plants as supplements to improve health conditions and quality of life in general.
Metrics
References
Hurrell R. (2003). Influence of vegetable protein sources on trace element and mineral availability. Journal of Nutrition, 133(9), 29733-29775.
Williams IO, Parker RS and Swanson J. (2009). Vitamin A content of South Eastern Nigerian vegetable dishes, their consumption pattern and contribution to vitamin A requirement. Pakistan Journal of Nutrition, 8(7), 1000-1004.
Hutchison J and Dalziel JM. (1973). Flora of West Tropical Africa (2nd ed.) Crown Agents, London, 638.
Bassey ME, Etuk EUI, Ubom R and Obop IE. (2006). Chemotaxonomic study of Lasianthera africana (lcacinaceae) in Akwa Ibom State of Nigeria. Nigeria Journal of Botany, 19, 99-102.
Sofowora, A. (1993). Screening plants for bioactive agents. Medicinal plants and traditional medicine in Africa, spectrum books Ltd, Ibadan, 134.
Ebana RUB, Essien Al and Ekpa OD. (1995). Nutritional and potential medicinal values of the leaves of Lasianthera africana (BEAUV). Global journal of pure science, 1, 1-8.
Nwosu MO and Malize N. (2006). An anatomic systematic study of medicinal plants of Nigeria Gongronema latifolium (benth) (Asclepladaceae). Journal of Economic and Taxonomic Botany, 30(2), 5-10
Ayodele AW. (2008). The medicinally important leafy vegetable of South Western Nigeria. http:llwww.siu/-ebl/leaflets/ayodele.com.
Ogunwande IA, walker TM, and Setzer WN. (2007). A review of aromatic herbal plants of Medicinal importance from Nigeria. Natural product communication, 2(12), 1311-1316.
Morebise O, Fafunso MA, Makinde JM, Olajide OA and Awe CO. (2002). Antiinflammatory property of the leaves of Gongronema latifolium. Phytotherapy Research, 16, 57-67.
Nwanjo HU, Okator MC, Oze GO. (2006). Antilipid peroxidative activity of Gongronema latifolium in streptozotocin induced diabetes rats. Nigeria Journal of Physiological Science, 221 (2), 61-65
Ebrahimzadeh MA, Hosseinimehr SJ, Hamidinia A and Jafari M. (2008a). Antioxidant and free radical scavenging activity of Feijoa sallowiana fruits peel and leaves. Pharmacologyonline 1, 7-9.
Ebrahimzadeh MA, Pourmorad F and Bekhradnia AR. (2008b). Iron chelating activity screening, phenol and flavonoid content of some medicinal plants from Iran. Africa Journal of Biotechnology, 32, 43-49.
Ebrahimzadeh MA, Pourmorad, F and Hafezi, S. (2008c). Antioxidant Activities of Iranian Corn Silk. Turkish Journal of Biology, 32, 43-49.
Lee SE, Hwany HJ, Ha JS, Jeong HS and Kin JH. (2003). Screening of medicinal plant extracts for antioxidant activity. Life science, 73,167-169.
Yen GH and Chen HY. (1995). antioxidant activity of various tea extracts in relation to their antimutagenicity. Journal of Agricultural and food chemistry, 43, 27-32.
Van Acker SA, Berg DJ, Tromp MN, Griffioen DH, Van Bennekom WP, van der Vijgh WJ and Bast A. (1996). Structural aspects of antioxidant activity of flavonoids. Free Radical Biology and Medicine, 20(3), 331-342.
Abdelwahab SI, Abdul AB, Elhassan MM, Mohan S Alzubairi AS and Mariod AA (2009a). Antimicrobial and free radical scavenging activities of dichloromethane extract of Goniothalamus umbrosus. International Journal of Tropical Medicine, 4, 32-36.
Mensor LL, Menezes FS, Leitao GG, Reis AS, Dos santos TC, Coube CS and Leitao SG. (2001). Screening of Brazilian plant extracts for antioxidant activity by the use of DPPH free radical method. Phytotherapy Research, 15, 127-130.
Hou WC, Lin RD, Cheng KT, Hung YT, Cho CH, Chen CH, Hwang SY and Lee MH. (2003). Free radical-scavenging activity of Taiwanese native plants. Phytomedicine, 10, 170-175.
Diplock AT. (1997). Will the good fairies please prove us that vitamin E lessens human degenerative diseases? Free radical research. 27, 511-532.
Heinonen IM, Lehtonen PJ and Hopia A. (1998). Antioxidant Activity of Berry and Fruit Wines and Liquors. Journal of Agricultural and Food Chemistry, 46, 25-31.
Wengs XC and Wang W. (2000). Antioxidant activity of various tea extracts in relation to their antimutagenicity. Journal of Agricultural and Food Chemistry, 43, 27-32.
Downloads
Published
How to Cite
Issue
Section
License
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
