Cytoprotective action of Griffonia simplicifolia (DC.) Baill. against the oxidative stress caused by hydrogen peroxide (H2O2) on neurons and astrocytes

Gueyraud Rolland Kipre; Shanker Pandey Hriday; M’baï Rostand Offoumou; Vini Tiwari; Diane Koulai; Priyanka Singh; Allico Joseph Djaman; Seth Pankaj

doi:10.30574/gscbps.2018.5.2.0093

Authors

Gueyraud Rolland Kipre
Shanker Pandey Hriday National Brain Research Centre, NH-8, Manesar, Gurugram, Haryana 122051, India.
M’baï Rostand Offoumou Laboratory of Botany, UFR Biosciences, Felix Houphouet-Boigny University, 22 BP 582 Abidjan 22 Ivory Coast.
Vini Tiwari National Brain Research Centre, NH-8, Manesar, Gurugram, Haryana 122051, India.
Diane Koulai Laboratory of Biochemical Pharmacodynamics, UFR Biosciences, Felix Houphouet-Boigny University, 22 BP 582 Abidjan 22 Ivory Coast.
Priyanka Singh National Brain Research Centre, NH-8, Manesar, Gurugram, Haryana 122051, India.
Allico Joseph Djaman Laboratory of Biochemistry, Pasteur Institute of Ivory Coast, 01 BP 490 Abidjan 01, Ivory Coast.
Seth Pankaj National Brain Research Centre, NH-8, Manesar, Gurugram, Haryana 122051, India.

DOI:

https://doi.org/10.30574/gscbps.2018.5.2.0093

Keywords:

Antioxidants, Cerebral malaria, Cytoprotective, Griffonia simplicifolia, Neurodegenerative diseases

Abstract

Cerebral malaria and neurodegenerative diseases such as Alzheimer's disease, Parkinson's disease and amyotrophic lateral sclerosis are characterized by a significant increase in oxidative stress that can lead to cellular damage to neurons and astrocytes. Therefore, the search for molecules capable of modulating oxidative stress in these diseases has recently gained interest, particularly for non-traditional antioxidants that can be obtained from plants. In this work, the aqueous leaf extract of Griffonia simplicifolia was used to evaluate its ability to protect both neurons and astrocytes against oxidative stress induced by hydrogen peroxide. The analysis of the chemical antioxidant activity showed that Griffonia simplicifolia has a good antioxidant activity with an IC₅₀ equal to 85.11 μg/mL. Toxicity tests showed that the aqueous extract of Griffonia simplicifolia did not affect the cellular viability of neurons and astrocytes (IC₅₀> 800 μg/mL). In addition, at a concentration of 20 μg/mL, the aqueous extract of Griffonia simplifica protected both neurons and astrocytes against H₂O₂-induced oxidative stress. Our results therefore suggest that the aqueous extract of Griffonia simplicifolia contains antioxidant molecules that may have therapeutic potential.

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