The difference of Lactobacillus vaginalis normal flora bacteria on vaginal swab on women at childbearing age using Piper betle L., soap, and water as vaginal cleanser

Authors

  • Sri Mudayatiningsih Malang State Health Polytechnic, Malang, Indonesia.
  • Endang Sri Dewi Hastuti Suryandari Malang State Health Polytechnic, Malang, Indonesia.

DOI:

https://doi.org/10.30574/gscbps.2018.5.3.0143

Keywords:

Cleanser, Lactobacillus vaginalis, Piper betle L., Water

Abstract

Hygiene and body care in women including genitalia’s organ holds a very vital role due to changes in pH balance, germs infection, physical factors, and the issue of self hygiene. Soap and Piper betle L. are types of genitalia cleanser which is often used by women. Piper betle L. contains phenol and tannin which can be used to treat female organs. Phenol is proven to be able to kill bacteria effectively including killing Candida albicans and Lactobacillus flora. Soaps containing triclosan which can damage certain enzyme synthesis in a cell can kill the pathogen bacterial bacteria even also the normal flora. Normal flora that resides in the female organs function to protect the area against fungal infection and other microorganism’s invasion of mucosal tissues of the vagina. Lactobacillus vaginalis is one of the normal vaginal flora with almost 95% of the normal vaginal flora, which produces lactic acid mixed with vaginal secretions resulting in acidic conditions in the vaginal area with a pH of 3.8-4.2. The purpose of this research was to prove that there is a difference in the number of normal bacteria Lactobacillus vaginalis on vaginal swab in women at childbearing age who use Piper betle L., soap and water as cleansers. This study is a descriptive comparative research with posttest only design. The analysis used t- test with a 95% confidence interval conducted on 30 respondents who were divided into three groups. The results showed that women at childbearing age who used Piper betle L. and soaps as vaginal cleanser experienced changes in their vaginal pH, so it was proven statistically that it can significantly decrease the number of Lactobacillus vaginalis.

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References

Rathee JS, Patro BS, Mula S, Gamre S and Chattopadhyay S. (2006). Antioxidant activity of Piper betel leaf extract and its constituents. Journal of agricultural and food chemistry, 54(24), 9046-9054.

Brooks GF, Butel JS and Morse SA. (2006). Medical microbiology. United States, 25th.

Boskey ER, Cone RA, Whaley KJ and Moench TR. (2001). Origins of vaginal acidity: high D/L lactate ratio is consistent with bacteria being the primary source. Human Reproduction, 16(9), 1809-1813.

Wesley AV and Margaret FW. (1993). Basics of Microbiology. Erlangga, Jakarta.

Nugent RP, Krohn MA and Hillier SL. (1991). (1991). Reliability of diagnosing bacterial vaginosis is improved by a standardized method of gram stain interpretation. Journal of clinical microbiology, 29(2), 297-301.

Sugiyono. (2008). Methodology of Quantitative and Qualitative and R&D Research. Penerbit Alfabeta. Bandung

Dahlan MS. (2009). Statistics for Health and Medical. Jakarta Salemba Medika.

Budiarto E. (2003). Methodology of medical research, an introduction. Penerbit Buku Kedokteran EGC. Jakarta

Brown JM, Poirot E, Hess KL, Brown S, Vertucci M and Hezareh M. (2016). Motivations for intravaginal product use among a cohort of women in Los Angeles. PLoS ONE, 11(3), e0151378.

Gustafsson RJ, Ahrné S, Jeppsson B, Benoni C, Olsson C, Stjernquist M and Ohlsson B. (2011). The Lactobacillus flora in vagina and rectum of fertile and postmenopausal healthy Swedish women. BMC women's health, 11(1), 17.

Mirmonsef P, Hotton AL, Gilbert D, Burgad D, Landay A, Weber KM, Cohen M, Ravel J and Spear GT. (2014). Free glycogen in vaginal fluids is associated with Lactobacillus colonization and low vaginal pH. PLoS One, 9(7), e102467.

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Published

2018-12-30

How to Cite

Mudayatiningsih, S., & Suryandari, E. S. D. H. (2018). The difference of Lactobacillus vaginalis normal flora bacteria on vaginal swab on women at childbearing age using Piper betle L., soap, and water as vaginal cleanser. GSC Biological and Pharmaceutical Sciences, 5(3), 066–070. https://doi.org/10.30574/gscbps.2018.5.3.0143

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Section

Short Comunication