The effect of ethanolic extract of Annona muricata leaf on the basal ganglia

Elizabeth Obioma Nweke; Frank Chinedu Akpuaka

doi:10.30574/gscbps.2019.6.3.0025

Authors

Elizabeth Obioma Nweke Department of Anatomy, Faculty of Basic Medical Sciences, Chukwuemeka Odumegwu Ojukwu University, Anambra State, Nigeria.
Frank Chinedu Akpuaka Department of Anatomy, Faculty of Basic Medical Sciences, Abia State University, Uturu, Abia State, Nigeria.

DOI:

https://doi.org/10.30574/gscbps.2019.6.3.0025

Keywords:

Annona muricata, Basal ganglia, Wistar rats, Histology

Abstract

Annona muricata (Linn.) is an evergreen tropical tree of the Annonaceae family that possesses phytotherapeutic bioactive compounds known as annonaceous acetogenins effective for the treatment of cancers and several diseases. This study investigated the effect of ethanolic extract of Annona muricata leaf on the basal ganglia. Twenty four (24) adult wistar rats were randomly divided into four groups (A, B, C & D) of six animals each. Group A served as the control and received 2 ml/kg of distilled water; the experimental groups B, C & D were orally administered 100 mg/kg, 200 mg/kg and 300 mg/kg body weight of ethanolic extract of Annona muricata leaf respectively for thirty days. At the end of administration, the animals were anaesthetized under the influence of chloroform vapour, dissected and their brain harvested for histological examination. The histological result revealed mild increase in the number of basal ganglia nuclei cell body (BGNCB) and fibres (F) with mild necrosis of neurons when compared to their control group. In conclusion, high consumption of ethanolic extract of Annona muricata could result in extrapyramidal side effects. Thus, it should be taken at lower doses.

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References

Reddy KJ. (2004). Medicinal plant research scenario in India, Info concepts India Inc. International Journal of Pharma Sciences and Research, 1, 25-28.

Keen RW, Deacon AC, Delves HT, Moreton JA and Frost PG. (1994): Indian herbal remedies for diabetes as a cause of lead poisoning. Postgraduate Medical Journal, 70, 113-114.

Adewole SO and Caxton-Martins EA. (2006). Morphological changes and hypoglycemic effects of Annona muricata Linn. (Annonaceae) leaf aqueous extract on pancreatic B-cells of streptozotocin-treated diabetic rats. African Journal of Biomedical Research, 9, 173–187.

De Souza R, Benassi E, da Silva RR, Afonso S and Scarminio IS. (2009). Enhanced extraction yields and mobile phase separations by solvent mixtures for the analysis of metabolites in Annona muricata L. Leaves. Journal of Separation. Science, 32, 4176–4185.

Misas C, Hernández N and Abraham A (1979). Contribution to the biological evaluation of cuban plants. Revista Cubana de Medicina Tropical, 31, 29-35.

Sundarrao K, Burrows I, Kuduk M, Yi YD, Chung MH, Suh NJ and Chang IM. (1993). Preliminary screening of anti-bacterial and anti-tumor activities of papuan new guinean active medicinal plants. Pharmaceutical Biology, 31, 3-6.

Heinrich M, Kuhnt M and Wright CW, (1992). Parasitological and microbiological evaluation of mixed Indian medicinal plants (Mexico). Journal of Ethnopharmacology, 36, 81-5.

Baskar R, Rajeswari V and Kumar TS. (2007). In vitro antioxidant studies in leaves of annona species. Indian Journal of Experimental Biology, 4, 480-5.

Antoun MD, Gerena L and Milhus WK (1993). Screening of the flora of Puerto-rico for potential antimalarial bioactives. International Journal of Pharmacology, 31, 255-8.

Leboeuf M, Cavé A, Bhaumik P, Mukherjee B and Mukherjee R. (1980). The phytochemistry of the annonaceae. Phytochemistry, 21, 2783–2813.

Kossouoh C, Moudachirou M, Adjakidje V, Chalchat JC and Figuérédo G. (2007). Essential oil chemical composition of Annona muricata L. Leaves from Benin. Journal of Essential Oil Research, 19, 307–309.

Chang FR, Liaw CC and Lin CY. (2003). New adjacent bis- tetrahydrofuran annonaceous acetogenins from Annona muricata. Planta Medica, 69, 241-6.

Mangal M, Khan MI and Agarwal SM. (2015). Acetogenins as potential anticancer agents. Anti-cancer agents in Medicinal Chemistry, 16(2), 138-159.

Zeng L, Wu FE and Oberlies NH, (1996). Five new monotetrahydrofuran ring acetogenins from the leaves of Annona muricata. Journal of Natural Products, 59, 1035-42.

Le Ven J, Schmitz-Afonso I, Touboul D, Buisson D, Akagah B, Cresteil T, Lewin G and Champy P. (2011). Annonaceae fruits and parkinsonism risk: metabolisation study of annonacin, a model neurotoxin; evaluation of human exposure. Toxicology Letters, 205:S50.

Lannuzel A, Hoglinger GU, Champy P, Michel PP, Hirsch EC and Ruberg M. (2006). Is atypical parkinsonism in the carribean caused by the consumption of Annonaceae? Journal of Neural Transmission, 70(70), 153-157.

Carparros-Lefebvre D and Elbaz A. (1999). Possible relation of atypical parkinsonism in the French West Indies with consumption of tropical plants: A case-control study. Lancet, 354(9175), 281-286.

Arthur FK, Terlabi EO, Larbie C and Woode E. (2012). Evaluation of hepatoprotective effect of aqueous extract of Annona muricata (Linn.) leaf against carbon tetrachloride and acetaminophen-induced liver damage. Journal of Natural Pharmaceuticals, 3, 25–30.

Escobar-Khondiker M, Höllerhage M, Muriel MP, Champy P, Bach A, Depienne C, Respondek G, Yamada ES, Lannuzel A and Yagi T. (2007). Annonacin, a natural mitochondrial complex I inhibitor, causes tau pathology in cultured neurons. Journal of Neuroscience, 27, 7827–7837.

Champy P, Melot A, Guérineau Eng V, Gleye C, Fall D, Höglinger GU, Ruberg M, Lannuzel A, Laprévote O and Laurens A. (2005). Quantification of acetogenins in Annona muricata linked to atypical Parkinsonism in guadeloupe. Movement Disorders, 20, 1629–1633.