Microbial quality of herbal preparations sold in some parts of Nigeria
DOI:
https://doi.org/10.30574/gscbps.2019.6.3.0031Keywords:
Herbal preparations, Microbial quality, Pathogenic bacteria, Bacillus spp.Abstract
The practice of using herbal drugs has become widespread in recent times without prior knowledge of their microbiological safety. In this context, this research was focused on assessing the microbial quality of different popular herbal preparations registered by the National Agency for Food and Drug Administration Control (NAFDAC) sold within South-West and South-South regions of Nigeria. The microbial diversity of some herbal drugs were examined by aerobic plate count method and microbial isolates were identified based on their morphological, cultural and biochemical characteristics. A total of sixty (60) herbal samples from six (6) herbal products (Kj cleanser, Ok Cleanser, Sk cleanser, Gk cleanser, Y-Bitters and Sw-Bitters) were analyzed and all were found to be free from pathogenic bacteria and indicator organisms of fecal contamination. However, Bacillus spp. was isolated from twenty six (26) of the herbal samples. Altogether four different Bacillus spp. were identified and the most predominant was Bacillus subtilis. The total heterotrophic bacterial count (THBC) of the herbal syrups ranged from 3.10 x102 cfu/ml to 2.65x103 cfu/ml whereas in the herbal powder the THBC ranged from 9.0x101 cfu/g to 1.5x102 cfu/g. The fungal count of herbal syrups ranged from 2.0x101cfu/ml to 1.9x102 cfu/ml while that of the herbal powder ranged from 1.0x101 cfu/ml to 1.0x102 cfu/ml. However, the microbial load and organisms present in the herbal samples were within WHO standard, therefore, it is recommended that plants to be used in herbal preparations should be properly washed and heated as to reduce or eliminate the presence of Bacillus species.
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References
Hack SK. (2005). Dot not put too much value on Conventional medicine. Ethnopharmacology, 3(2), 37-39.
Okeke, IN, Lamikanra A and Edelman H. (1999). Socioeconomic and behavioural factors leading to acquired bacterial resistance to antibiotics in developing countries. Emerging Intestine Diseases, 2(5), 18-27.
WHO (1998), Quality control methods for medicinal plant materials. World Health Organization, Geneva, 112-113.
Oyetayo VO. (2008). Microbial load and Antimicrobial Property of two Nigerian Herbal Remedies Africa. Journal of Trade Comparative Medicine, 5, 74-76.
Kasilo OMJ and Trapsida JM. (2011). Decade of African traditional medicine 2001-2010. Afr, Health Mon (Special issue), 14, 25-31.
Zhang X. (1998). Regulatory situation of herbal medicines: a worldwide review. 2003: World Health Organization. Available at, http://apps.who.int/ medicinedocs/en/d/Jwhozip57e/, (accessed 8 June 2012).
Ali N, Hashim NH, Saad B, Safan K, Nakajima M and Yoshizawa T. (2005). Evaluation of a method to determine the natural occurrence of aflatoxins in commercial traditional herbal medicines from Malaysia and Indonesia. Food Chemistry and Toxicology, 43(12), 1763-1772.
Sharafati R, Sharafati F and Rafieian-kopaei M. (2011). Biological characterization of Iranian walnut (Juglans regia) leaves. Turkish Journal of Biology, 35, 635-639.
Lau A, Holmes MJ, Woo S and Koh H. (2003). Analysis of adulterants in traditional herbal medicinal product using liquid chromatography – mass spectroscopy. Journal of Pharmaceutical and Biological Anatomy, 1(2), 31-40.
Muinde OK and Kuria E. (2005). Hygienic and Sanitary Practices of Vendors of Street Foods in Nairobi, Kenya. African Journal of Food Agriculture and National Development, 5(1).
John HG, Krieg NR, Sneath PHA, Stanley JT and Willians ST. (1994). Bregey’s Manual of Determinative Microbiology. 9th edition, Baltimore: Williams and Wilkins.
Abba D, Inabo HI, Yakubu SE and Olonitola OS. (2009). Contamination of herbal medicinal products marketed in Kaduna Metropolis with selected pathogenic bacteria. African Journal of Traditional, Complementary and Alternative Medicines, 6, 70.
Kneifel W, Czech E and Kopp B. (2002). Microbial contamination of medicinal plants- A review. Planta Medica, 5-15, 68.
Bugno A, Almodovar AAB, Pereira TC, Pinto TJA and Sabino M. (2006). Occurrence of toxigenic fungi in herbal drugs. Brazilian Journal of Microbiology, 37, 47-51.
Martins HM, Martins MI, Dias M. BernardoF I. (2001). Evaluation of microbiological ```quality of medicinal plants used in natural infusions. International Journal of Food Microbiology, 68, 149-1533.
Shah B and Pokhrel N. (2012). Microbial Quality and Antimicrobial Activity of Herbal medicine. Nepal Journal of Science and Technology, 13(2), 191-196.
Esimone CO, Chah KF and Ikejide SC. (2002). Microbiological quality of Herbal preparations marketed in South-East Nigeria. Journal of Natural Remedies, 2(1), 42-48.
Okunlola A, Adewoyin BA and Odeku OA. (2007). Evaluation of Pharmaceutical and Microbial Qualities of some Herbal Medicinal Products in South Western Nigeria. Journal of Pharmaceutical and Biological Anatomy, 2(3), 661-670.
Adeleye IA, Okogi G and Ojo EO. (2005). Microbial contamination of herbal preparation in Lagos, Nigeria. Journal of Health, Population and Nutrition, 23(3), 296-297.
Anyanwu CU. (2010). Fungal contaminants of powdered herbal drugs sold in parts of Enugu State, Southeast, Nigeria. Plant Product Research Journal, 14, 46 -50
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