Haematological profile of albino rats exposed to polar leaf extracts of Portulaca oleracea Linn.

Victoria Chinenye  Obinna; Hope Delesi Kagbo; Ozadheoghene Eriarie Afieroho; Gabriel Ogaba Agu

doi:10.30574/gscbps.2019.7.1.0042

Authors

Victoria Chinenye Obinna Department of Animal and Environmental Biology, Faculty of Science, University of Port Harcourt, Rivers State, Nigeria.
Hope Delesi Kagbo Department of Pharmacology, Faculty of Basic Medical Sciences, University of Port Harcourt, Rivers State, Nigeria.
Ozadheoghene Eriarie Afieroho Department of Pharmacognosy and Phytotherapy, Faculty of Pharmaceutical Sciences, University of Port Harcourt, Rivers State, Nigeria.
Gabriel Ogaba Agu Department of Animal and Environmental Biology, Faculty of Science, University of Port Harcourt, Rivers State, Nigeria.

DOI:

https://doi.org/10.30574/gscbps.2019.7.1.0042

Keywords:

Portulaca oleracea, Haematology, Polar extract, Aqueous methanol, Phytochemical

Abstract

Although the use of medicinal plants is becoming popular globally, some of these plants which are purported to be safe are not without side effects or toxicity. Portulaca oleracea, Linn. is among the medicinal plants used globally in the treatment of diseases and management of health challenges. The dearth of information on the toxicity of Portulaca oleracea in a long term use prompted this study which investigated the sub-chronic effect of the oral administration of polar (aqueous methanol) leaf extracts of Portulaca oleracea on haematological parameters in male albino rats. Sixty-four animals were randomly divided into 4 groups of 16 rats each. Group 1(Control) received 0.5 ml of 20% Tween 80 (vehicle), Groups 2, 3 & 4 received 125, 250 & 500 mg/kg bw of the extract respectively for 60 days by oral gavage. On days 14, 28, 42 and 60; four rats from each group were anaesthetized and blood samples were collected for haematology. No significant (p>0.05) variation occurred in the mean values of PCV, hemoglobin concentration, RBC count, platelet count and differential leucocyte count relative to the control throughout the 60-day duration. Significant (p<0.05) increase in WBC count was recorded on day 42 in the 500mg treated group. Oral administration of polar leaf extract of P.oleracea as used in this study had no injurious effect on haematological parameters in a long term treatment of 60 days; thus can be said to be non-toxic to blood parameters.

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References

Bala K, Mahima A and Deepshikha PK. (2014). Herbal Contraceptive: An Overview. World Journal of pharmacy and pharmaceutical science, 3(8), 1305-1326.

Modaresi M and Khodadadi A. (2014). The effects of Aloe vera extract on reproductive parameters in mice. International Conference on Biological, Environment and Food Engineering (BEFE-2014) August 4-5, Bali (Indonesia).

Mitich LW. (1997). Common Purslane (Portulaca oleracea), Weed Technology, 11 (2), 394-397.

Burkill HM. (1985). The useful plants of West Tropical Africa, second edition Vol 1. Royal Botanic Garden, Kew, 960.

Iwu M. (1993). Handbook of African Medicinal Plants, second edition. Boca Raton, CRC Press, 464

Shafi S and Tabassum N. (2015a). Preliminary phytochemical screening, Renal and Haematological effects of Portulaca oleracea (Whole Plant) in swiss albino mice. International Research Journal of Pharmacy, 6(6), 349-353

Nayaka HB, Londonkar RL, Asha TNK, Sanjeev Kumar CB and Sudarshan M. (2014). In vitro cytotoxic and gene toxicity effects of flavonoid of Portulaca oleracea L. on HepG2 and K562 malignant cell lines. International Journal of Basic Applied Biology, 2(1), 89 – 93

Foster S and Duke JA. (2000). A field guide to medicinal plants and herbs, Second edition. Boston, Houghton Mifflin Company, 1657

Quisumbing E. (1978). Medicinal Plants of the Philippines. Philippines, Katha Publishing Company. JMC PRESS, Quezon City,

Chen J, Shi YP and Liu JY (2003). Determination of noradrenaline and dopamine in Chinese herbal extracts from Portulaca oleracea L. by high-performance liquid chromatography. Journal of Chromatography, 1003, 127–132

Samarghandian S, Borji A and Farkhondeh T. (2017). Attenuation of oxidative stress and inflammation by Portulaca oleracea in streptozotocin-induced diabetic rats. Journal of Evidence-based Complementary Alternative Medicine, 22(4), 562-566.

Bai Y, Zang X, Ma J and Xu G (2016). Antidiabetic effect of Portulaca oleracea L. polysaccharide and its mechanism in diabetic rats. International Journal of Molecular Science, 17(8), 1201.

Allam A, Juraimi AS, Rafii MY, Hamid AA, Aslani F, Zainudin MAM, et al. (2014). Evaluation of antioxidant compounds, antioxidant activities and mineral composition of 13 collected purslane (Portulaca olearcea L.) Accessions. Biomedical Research International, Article ID 296063, 10 pages.

Uddin K, Juraimi AS, Hossain S, Nahar A, Ali E and Rahman MM (2014). Purslane weed (Portulaca oleracea): A prospective plant source of nutrition, omega-3-fatty acid, and antioxidant attributes. The Scientific World Journal, Article ID 951019, 6 pages.

Rashed AN, Afifi FU and Disi AM. (2003). Simple evaluation of the wound healing activity of a crude extract of Portulaca oleracea (growing in Jordan) in Mus musculus JV1-1. Journal of Ethnopharmacology, 88, 131-136.

Harborne JB. (1998). Phytochemical methods- a guide to modern techniques of plant analysis, third edition. London, Chapman and Hall.

Houghton PJ and Raman A. (1999). Laboratory Handbook for the Fractionation of Natural Extracts. London, Chapman and Hall

Lorke D. (1983). A new approach to practical acute toxicity testing. Archives of Toxicology, 54(4), 275-87.

Cheesebrough M. (2006). District Laboratory Practice in tropical countries. Cambridge University Press, 131-132.

Pandey A and Tripathi S. (2014). Concept of standardization, extraction and pre phytochemical screening strategies for herbal drug. Journal of Pharmacognosy and Phytochemistry, 2 (5), 115-119.

Widyawati PS, Budianta TDW, Kusuma FA and Wijaya EL. (2014). Difference of solvent polarity to phytochemical content and antioxidant activity of Pluchea indicia Less leaves extracts. International Journal of Pharmacognosy and Phytochemical Research, 6(4), 850-855

Yu Lin H, Kuo YH, Lin YL and Chiang W. (2009). Antioxidative effect and active component from leaves of lotus (Nelumbo nucifera). Journal of Agriculture and Food Chemistry, 57, 6623 -6629.

Dehkharghanian M, Adenier H and Vijayalakshmi MA. (2010). Analytical methods study of flavonoids in aqueous spinach extract using positive electrospray ionisation tandem quadrupole mass spectrometry. Food Chemistry, 121, 863 - 870.

Cowan MM. (1999). Plant product as antimicrobial agents. Clinical Microbiology Reviews, 12(4), 564 - 582.

Schalm OW, Jain NC and Caroll EJ. (1975). Veterinary Haematology, third edition. Philadelphia, Lea and Febiger 15-28

Togun VA, Oseni BSA, Ogundipe JA, Arewa TR, Hammed AA, Ajonijebu DC, et al. (2007). Effects of chronic lead administration on the haematological parameters of rabbits – a preliminary study. Proceedings of the 41st Conferences of the Agricultural Society of Nigeria, 341

Brosche T, Platt D and Dorner H. (1990). The effect of garlic preparation on the composition of plasma lipoproteins and erythrocyte membranes in geriatric subjects. British Journal of Clinical Practice, 69, 12-19.

Obinna VC and Kagbo HD. (2018). Haematological profile of rat offspring exposed to beta cypermethrin during the perinatal period. Saudi Journal of Medicine and Pharmaceutical Science, 4(1B), 156-159.

Peters SO, Gunn HH, Imumorin IG, Agaviezor BO and Ikeobi CO. (2011). Haematological studies on frizzled and naked neck genotypes of Nigerian native chickens. Tropical Animal Health Prouction, 43(3), 631-638.

Guha G, Rakumar v, Ashok Kumar R and Mathew L (2011). Therapeutic potential of polar and non-polar extracts of Cyanthillium cinerum in vitro. Evidence-Based Complementary and Alternative Medicine Volume Article ID 784826, 10 pages.

Smith L. (2018). What’s to know about high white blood cell count? https://www.medicalnewstoday.com/articles/315133.phpAccesed1st August 2018.

Soetan KO, Akinrinde AS and Ajibade TO. (2013). Preliminary studies on the haematological parameters of cockerels fed raw and processed guinea corn (Sorghum bicolor) Proceedings of 38th Annual Conference of Nigerian Society for Animal Production, 49-52.

Oyedeji KO and Bolarinwa AF. (2012). Effects of crude extracts of Portulaca oleracea on haematological and biochemical parameters in albino rats. African Journal of Biomedical Research, 15, 41– 47

Shafi S and Tabassum N (2015b). Toxicity Evaluation of Hydro-Alcoholic extract of Portulaca oleracea (Whole Plant) in swiss albino mice. International Journal of Pharmacy and Pharmaceutical Science, 7(2), 506-510.

Ezeabara, CA, Ikeh CF, Ilodibia CV, Aziagba BO, Okanume OE and Mbaekwe EI (2014). Comparative determination of phytochemical, proximate and mineral compositions in various parts of Portulaca oleracea Linn. Journal of Plant Science, 2(6), 293-298.

Obinna VC, Kagbo HD and Agu GO. (2018). Hepatic and Renal Biochemical profile of albino rats exposed to chloroform and methanol leaf extracts of Portulaca oleracea Linn. European Journal of Medicinal Plants, 25(3), 1-14.