Analytical method validation for metformin quantification in dissolution medium

Joselyn Bogantes-Molina; German Leonardo Madrigal-Redondo; Lorena Berrocal-Barrantes; Marianela Chavarría-Rojas; Daniel Alejandro Acuña-Amador; Rolando Vargas-Zúñiga

doi:10.30574/gscbps.2019.7.2.0049

Authors

Joselyn Bogantes-Molina Pharmacy School, Universidad Latina of Costa Rica, San José, Costa Rica.
German Leonardo Madrigal-Redondo Pharmacy School, Universidad Latina of Costa Rica, San José, Costa Rica.
Lorena Berrocal-Barrantes Pharmacy School, Universidad Latina of Costa Rica, San José, Costa Rica.
Marianela Chavarría-Rojas Pharmacy School, Universidad Latina of Costa Rica, San José, Costa Rica.
Daniel Alejandro Acuña-Amador Faculty of Pharmacy, University of Costa Rica, San José, Costa Rica.
Rolando Vargas-Zúñiga Faculty of Pharmacy, University of Costa Rica, San José, Costa Rica.

DOI:

https://doi.org/10.30574/gscbps.2019.7.2.0049

Keywords:

Biological and Pharmaceutical Sciences, Validation, Analytical method, Quantification, Dissolution, Spectrophotometry

Abstract

Metformin hydrochloride is a hypoglycemic agent used for type II Diabetes Mellitus treatment, and one of the most used to manage it. The objective of the present work was the development and validation of an analytical method to quantify metformin hydrochloride in the dissolution medium by UV spectrophotometry. Linearity and range, accuracy and precision were the validation process parameters. Validation process results showed the analytical method was easy, quick, secure and, furthermore, a linear, accurate and precise method in the studied concentrations range. Therefore, it is a reliable analytical method.

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References

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