Phytochemicals and chelating properties in extract of Celosia trigyna inhibits xanthine oxidase in vitro

AS Eboh; EM Arhoghro; A Frank-Oputu; E Wodu; OG Adah; BA Uwuma

doi:10.30574/gscbps.2019.8.2.0064

Authors

AS Eboh Biochemistry Department, Niger Delta University, Bayelsa State, Nigeria.
EM Arhoghro Biochemistry Department, Niger Delta University, Bayelsa State, Nigeria.
A Frank-Oputu Biochemistry Department, Niger Delta University, Bayelsa State, Nigeria.
E Wodu Biochemistry Department, Niger Delta University, Bayelsa State, Nigeria.
OG Adah Biochemistry Department, Niger Delta University, Bayelsa State, Nigeria.
BA Uwuma Biochemistry Department, Niger Delta University, Bayelsa State, Nigeria.

DOI:

https://doi.org/10.30574/gscbps.2019.8.2.0064

Keywords:

Celosia trigyna, Total flavonoid, Vitamin C, Phenolic acid;, Xanthine oxidase

Abstract

This study is designed to investigate the inhibitory effect of Celosia trigyna against xanthine oxidase. Total flavonoid, phenol, vitamin C were evaluated using standard methods and inhibitory activity of Celosia trigyna extract on xanthine oxidase (XO) activity in vitro and chelating properties of extract against Iron (II) were also carried out. The results showed that the extract contained high content of flavonoids, phenolic acid and vitamin C that are beneficial to health. The extract inhibited XO in a dose-dependent manner. The extract also chelated Fe2+ in a dose-dependent manner. Celosia trigyna extract could be a promising nutraceutical for preventing and managing hyperuricaemia due to its ability to inhibit XO; this activity is attributed to the combined effect of its phytochemicals and chelating properties.

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References

Borges F, Fernandes E and Roleira F. (2002). Progress towards the discovery of xanthine oxidase inhibitors, Curr. Med. Chem, 9 (2), 195–217

Haidari F, Rashidi MR and Keshavarz SA. (2008). Effects of onion on serum uric acid level and hepatic xanthine dehydrogenase/xanthine oxidase activities in hyperuricemic rats. Pakistan Journal of Biological Sciences. 11(14), 177-180.

Varadharaj V. (2017). Phytochemical and phytotherapeutic properties of celosia argentea. International Journal of Pharmacy and Pharmaceutical Research, 9(6), 20-25.

Babatola JO and Ogunware JB. (1978). Effects of the root-knot nematode, Meloidogyne incognita on growth and yield of three local vegetables. Proceedings of the first Annual Conference Horticultural Society of Nigeria; 1978, 1–3 November. Ibadan, Nigeria, 15–105.

Burkill HM. (1985). The useful plants of West Tropical Africa. 2nd Edition. Volume 1, Families A–D. Royal Botanic Gardens, Kew, Richmond, United Kingdom. 960.

Public Health Service (PHS). Public Health Service Policy on Humane Careand Use of Laboratory Animals, (PL 99-158. Health Research ExtensionAct, 1985), US Department of Health and Human Services, Washington, DC, 1996.

Umamaheswari M, Kumar KA, Somasundaram A, Sivashanmugam T, Subhadradevi V and Ravi TK. (2007).Xanthine oxidase inhibitory activity of some Indian medical plants, J. Ethnopharmacol, 109, 547–551.

Singleton P. (1999) Bacteria in biology, biotechnology and medicine. 4th ed. NewYork: JohnWiley and Sons Ltd, 324–37.

Demiray S, Pintado ME, and Castro PM. (2009).Evaluation of phenolic profiles and antioxidant activities of Turkish medicinal plants: Tilia argentea, Crataegi folium leaves and Polygonum bistorta roots. World Acad Sci Eng Technol, 54, 312–17.

Zhishen Y, Meugcheng T, Jianming W. (1999). Determination of flavonoids content in mulberry and their scavenging effect on superoxide radicals. Food Chem, 64, 555–9.

Klein BP and Perry AK. (1982). Ascorbic acid and vitamin A activity in selected vegetables from different geographical areas of the United States. Journal of Food Science, 47, 941–945.

Minotti G, Aust SD. (1987). An investigation into the mechanism of citrate-Fe2+-dependent lipid peroxidation. Free RadicBiol Med, 3, 379–87.

Puntel RL, Nogueira CW, Rocha JBT. (2005). Krebs cycle intermediates modulate thiobarbituric reactive species (TBARS) production in rat brain in vitro. Neurochem Res, 30, 225–35.

Balasundram N, Sundram K and Samman S. (2006) Phenolic compounds in plants and agri-industrial by-products: Antioxidant activity, occurrence, and potential uses. Food Chem, 99, 191-203.

Anila L and Vijayalakshmi NR. (2003). Antioxidant action of flavonoids from Mangifera indica and Emblica officinalis in hypercholesterolemic rats. Food Chem, 83, 569-574.

Barros AIRNA, Nunes FM, Goncalves B, Bennett RN and Silva AP. (2011). Effect of cooking on total vitamin C contents and antioxidant activity of sweet chestnuts (Castanea sativa Mill.). Food Chem. 128, 165–172.

Rice-Evans CA, Miller NJ and Paganga G. (1996). Structure–antioxidant activity relationships of flavonoids and phenolic acids. Free Radic. Biol. Med. 20, 933–956.

Miller DM, Buettner GR and Aust SD. (1990). Transition metals as catalysts of "autoxidation" reactions. Free Radic Biol Med., 8, 95–108.