Protective effects of vitamin E and grape seed oil against acute hepatorenal ivermectin toxicity in mice: biochemical and histopathological studies

Maghraby Mai Mohsen El; Maghraby Nada Mosaad El; Ameera Ahmed Salama; Azza Shawky Abdlmonem; Eman Abdellatefe Authman; Esraa Abdelraouf Abdelmohsen; Mohamed Abd-Elreheem  Eltras; Omnia Nasr Barseem; Shimaa Refat Awad; Ahmed Ashraf Matter; Sleam Ahmed Magdy Abo; Esraa Ahmed Elgozier; Mohamed Ali Elnagar; Bialy Badr El Said El; Borai Nermeen Borai El

doi:10.30574/gscbps.2019.7.2.0079

Authors

Maghraby Mai Mohsen El Undergraduate Student, Fifth Academic Year, Faculty of Veterinary Medicine, University of Sadat City, Sadat City, Egypt.
Maghraby Nada Mosaad El Undergraduate Student, Fifth Academic Year, Faculty of Veterinary Medicine, University of Sadat City, Sadat City, Egypt.
Ameera Ahmed Salama Undergraduate Student, Fourth Academic Year, Faculty of Veterinary Medicine, University of Sadat City, Sadat City, Egypt.
Azza Shawky Abdlmonem Undergraduate Student, Fourth Academic Year, Faculty of Veterinary Medicine, University of Sadat City, Sadat City, Egypt.
Eman Abdellatefe Authman Undergraduate Student, Fourth Academic Year, Faculty of Veterinary Medicine, University of Sadat City, Sadat City, Egypt.
Esraa Abdelraouf Abdelmohsen Undergraduate Student, Fourth Academic Year, Faculty of Veterinary Medicine, University of Sadat City, Sadat City, Egypt.
Mohamed Abd-Elreheem Eltras Undergraduate Student, Fourth Academic Year, Faculty of Veterinary Medicine, University of Sadat City, Sadat City, Egypt.
Omnia Nasr Barseem Undergraduate Student, Fourth Academic Year, Faculty of Veterinary Medicine, University of Sadat City, Sadat City, Egypt.
Shimaa Refat Awad Undergraduate Student, Fourth Academic Year, Faculty of Veterinary Medicine, University of Sadat City, Sadat City, Egypt.
Ahmed Ashraf Matter Undergraduate Student, Fourth Academic Year, Faculty of Veterinary Medicine, University of Sadat City, Sadat City, Egypt.
Sleam Ahmed Magdy Abo Undergraduate Student, Fourth Academic Year, Faculty of Veterinary Medicine, University of Sadat City, Sadat City, Egypt.
Esraa Ahmed Elgozier Undergraduate Student, Fourth Academic Year, Faculty of Veterinary Medicine, University of Sadat City, Sadat City, Egypt.
Mohamed Ali Elnagar Undergraduate Student, Fourth Academic Year, Faculty of Veterinary Medicine, University of Sadat City, Sadat City, Egypt.
Bialy Badr El Said El Department of Forensic Medicine and Toxicology, Faculty of Veterinary Medicine, University of Sadat City, Sadat City, Egypt.
Borai Nermeen Borai El Department of Forensic Medicine and Toxicology, Faculty of Veterinary Medicine, University of Sadat City, Sadat City, Egypt.

DOI:

https://doi.org/10.30574/gscbps.2019.7.2.0079

Keywords:

Ivermectin, Vitamine E, Grape seed oil, Hepatorenal toxicity, Histopathology

Abstract

Ivermectin (IVM), a broad spectrum anthelmintic drug, is considered a safe drug; however, there are few reports about its toxic effects in particularly at accidental overdose exposure. Therefore, the current study was designed to investigate the potential protective roles of vitamin E (Vit E) and grape seed oil (GSO) against the acute hepatorenal toxicity of IVM in mice. Mice were divided into four equal groups. Control vehicle group was administrated corn oil (0.2 ml/animal), IVM group was administrated IVM (6.5 mg/kg b.w.), Vit E+IVM group was administrated Vit E (200 mg/kg b.w.) plus IVM and GSO+IVM group was administrated GSO (0.2 ml/animal) plus IVM. All treatments were orally administrated daily for 3 weeks while IVM is administered as a single oral dose one day before the end of the experiment. The results revealed that IVM induced significant elevation in serum ALT and AST activities; urea and creatinine levels without any significant change in glucose level. No marked histopathological alterations were observed in hepatic tissue with several pathological alterations in the kidneys of IVM- intoxicated mice. However, pretreatment of mice with either Vit E or GSO ameliorated the IVM- induced biochemical and histopathological alterations. In conclusion, Vit E and GSO may provide a similar promising protective value against IVM acute hepatorenal intoxication

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