Propagation spores of arbuscular mycorrhiza fungi and rooting colonization characteristic’s on different host plants

Halim; Jaya Arma Makmur; Sarawa; Corina Rakian Tresjia; Tufaila Muhammad; Resman; Suramas Rembon Fransiscus; Siti Anima Hisein Waode; Syair; Mariadi; Mane Kandari Aminuddin

doi:10.30574/gscbps.2019.8.1.0114

Authors

Halim Department of Agrotechnology. Halu Oleo University, Southeast Sulawesi, Indonesia.
Jaya Arma Makmur Department of Agrotechnology. Halu Oleo University, Southeast Sulawesi, Indonesia.
Sarawa Department of Agrotechnology. Halu Oleo University, Southeast Sulawesi, Indonesia.
Corina Rakian Tresjia Department of Agrotechnology. Halu Oleo University, Southeast Sulawesi, Indonesia.
Tufaila Muhammad Department of Soil Science, Halu Oleo University, Southeast Sulawesi, Indonesia.
Resman Department of Soil Science, Halu Oleo University, Southeast Sulawesi, Indonesia.
Suramas Rembon Fransiscus Department of Soil Science, Halu Oleo University, Southeast Sulawesi, Indonesia.
Siti Anima Hisein Waode Department of Plant Protection, Faculty of Agriculture, Halu Oleo University, Southeast Sulawesi, Indonesia.
Syair Department of Plant Protection, Faculty of Agriculture, Halu Oleo University, Southeast Sulawesi, Indonesia.
Mariadi Department of Plant Protection, Faculty of Agriculture, Halu Oleo University, Southeast Sulawesi, Indonesia.
Mane Kandari Aminuddin Department of Environmental Science, Faculty of Forestry and Environmental Science, Halu Oleo University, Southeast Sulawesi, Indonesia.

DOI:

https://doi.org/10.30574/gscbps.2019.8.1.0114

Keywords:

Acaulospora sp, Glomus sp, Host plants, Root colonization, Spores abundance

Abstract

This research aimed to study the propagation spores of arbuscular mycorrhiza fungi (AMF) on different host plants. This research conducted in completely randomized block design (CRBD) with factorial pattern and each treatment was repeated three times and each consist five pot cultures, the total of treatments are 150 pot culture. The first factor are: kinds of host plant (A) with five levels i.e.: Pueraria javanica (A₁), Centrocema pubescens (A₂), Sorgum bicolor (A₃), Cajanus cajan (A₄), Zea mays (A₅) and second factor are mycorrhyza fungi (B) which comprises two levels i.e.: Glomus sp (B₁), Acaulospora sp (B₂. The result of research showed that the highest average of spores number on P. javanica + Glomussp as 38 spores and lowest on C. pubescens+ Glomus sp and Z. mays + Glomus sp respectively as 28 spores. The percentage of roots colonization for Glomus sp on kinds of host plant i.e.: P. javanica as 60.36%, S. bicolor as 47.93%, Z. mays as 35.36%, C. cajan as 21.72%, C. pubescens as 20.55%. The percentage of roots colonization for Acaulospora sp on S. bicolor as 39.83%, C. cajan as 27.98%, C. pubescens as 26.57%, P. javanica as 23.82%, Z. mays as 21.20%. The highest average weight of shoots and roots on treatment of P. javanica (A₁) each 0.82 g and 0.91 g.

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References

Brundrett M, Bougher N, Dell B, Grove T, and Malajczuk. (1996). Working with mycorrhizas in forestry and agriculture. Australian Centre for International Agricultural Research Mohograph, 372-374.

Smith SE and Read DJ. (1997). Mycorrhizal Symbiosis. Second Edition. Academiz Press. Harcourt Brace & Company Publisher. London.

Hazard C, and Johnson D. (2007). Does genotypic and species diversity of mycorrhizal plants and fungi affect ecosystem function? Tansley Insight. New Phytologist, 1-7.

Halim, Karimuna L, Rembon FS, Resman and Supriatun T. (2016a). Growth and potential of goat weed (Ageratum conyzoides L.) as host plant for propagation of mycorrhiza fungi. Open Access Library Journal, 3(e2640), 2-8.

Gunawan AW. (2003). Mikoriza Arbuskula. Pusat Antar Universitas Ilmu Hayat. Institut Pertanian Bogor. Indonesia.

Bakhtiar Y. (2002). Selection of vascular mycorrhiza (VAM) fungi. Host plants and spore numbers for producing inoculum. J. Biosains and Bioteknologi Indonesia, 2(1), 36-40.

Casazza G, Lumini L, Ercole E, Dovana F, Guerrina M, Arnulfo A, Minuto L, Fusconi, A and Mucciarelli M. (2017). The abundance and diversity of arbuscular mycorryzal fungi are linked to the soil chemistry of screes and to slope in the Alpic paleo-endemic Berardia subacaulis. PLOS ONE, 12(2), e0171866.

Bagyaraj DJ. (1992). Ecology of Vesicular-arbuscular mycorrhizae. Hand Book of Applied Mycology. Soil and Plants. New York, Marcel Dekker.

Irianto RSB. (2009). The technique of producing arbuscular mycorrhizal fungi Glomus etunicatum in sorghum and pueraria plants. Forest Research and Development Center and Nature Conservation. Balitbang Kehutanan Ministry of Forestry. Bogor.

The technique of producing arbuscular mycorrhizal fungi Glomus etunicatum in sorghum and pueraria plants. Forest Research and Development Center and Nature Conservation. Balitbang Kehutanan Ministry of Forestry. Bogor, 6(1), 83-87.

Prematuri R and Faiqoh N. (1999). Production of arbuscular mycorrhizal fungi inoculum. Forest Biotechnology Laboratory, Biotechnology PAU IPB. Bogor.

Sieverding E. (1991). Vesicular-arbuscular mychorrhyza management in tropical agrosystems. Technical Cooperation Federal Republic of Germany. Eschborn.

Halim, Arma MJ, Rembon FS and Resman. (2015). Impact of mycorrhiza fungi from grassland rhizosphere and liquid organic fertilizer to the growth and yield of sweet corn on Ultisols in South Konawe, Indonesia. International Journal of Agriculture, Forestry and Fisheries, 4(5), 209-215.

Halim, Arma MJ, Karimuna L, Rembon FS and Resman. (2016). Effect of mycorrhiza fungi propagules and bokashi fertilizer combination to weed density, growth and yield of maize (Zea mays L.) on marginal dry land in Regency of Kendari, Indonesia. International Journal of Development Research, 6(9), 9568-9575.

Halim. (2009). The role endomycorrhiza indigenous of Imperata cylindrica (L.) Beauv and Eupatorium odorata (L.) in weed and corn competitive. (Dissertation). Padjadjaran University, Bandung. Indonesia, 52-128.

Halim, Rembon FS, Kandari AM, Resman and Sani A. (2014). Characteristics of indigenous mycorrhiza of weeds on marginal dry land in South Konawe, Indonesia. International Journal of Agriculture, Forestry and Fisheries, 3(6), 459-463.

Halim, Supriatun T, Karimuna L, Hasid R, Rembon FS and Mariadi. (2016). Impact of mycorrhiza fungi isolated from weed plants on growth of pepper plant seedling (Piper nigrum L.) and incidence of stem rot desease (Phytophthora capsici) in Net House Treatment. International Journal of Current Research, 8(05), 31419-31426.

Berruti A, Lumina E, Balestrini R and Bianciotto V. (2016). Arbuscular mycorrhiza fungi as natural biofertilizer: Let’s benefit from past success. Frontier in Microbiology, 6, 1559.

Mosee B. (1981). Vesicular-arbuscular mychorrhyza research for tropical agriculture. Res. Bull. 194. Hawaii Institut for Tropical Agriculture.

Kim SJ, Eo JK, Lee EH, Park H and Eom AH. (2017). Effect of arbuscular mycorrhizal fungi and soil conditions on crop plant growth. Mycobiology, 45(1), 20-24.

Carrenho R, Trufem SFB and Bononi VLR. (2002). Effects of using different host plants on the detected biodiversity of arbuscular mycorrhiza fungi from an agroecosystem. Revita Brasil.Bot, 25(1), 93-101.

Buce M, Rossignol M, Jauneau A, Ranjeva R and Beacard G. (2000). The presymbiotic growth of arbuscula mycorrhizal fungi is induced by a branching factor partially purified plant root exudates. Mol. Plant. Nederlands Institute of Ecology.

Brundrett, M and Kendrick, B. (1990). The root and mycorrhizas of herbaceous woodland plant. II. Structural Aspects of Morphology. New Phytologist, 114, 469-479.

Sancayaningsih RP. (2005). The effects of single and dual inoculations of arbuscular mycorhhizal fungi on plant growth and the EST and MDH enzyme profiles of maize roots (Zea mays L.) Grown on Limited Growth Media (Dissertation). UGM. Yogyakarta.

Brundrett M. (1999). Arbuscular Mycorrhizas. CSIRO Forestry and Forest Products.

Barea JM. (1991). Vesicular-arbuscular mycorrhizas as modifiers of soil fertility. Adv. Soil Science.

Juge C, Samson J, Bestien C, Vierheilig H, Coughlan A and Pieche Y. (2002). Breaking dormancy in spores of the arbuscular mycorrhizal fungus Glomus intraradies. A critical cold storage period. Journal of Mycorrhiza, 12, 37-42.

Goltapeh EM, Danesh, Prasad, YR and Varma A. (2008). Mycorrhizal Fungi. Genetic and molecular biology, Eco-Function, Biotechnolgi. Eco-Physiology. Springer-Verlag. Berlin Heidenberg.

Pearson VG, Tollot M and Seddas PMA. (2009). Dissection of genetic cell programs driving early arbuscular mycorrhiza interactions, in Aguilar CA, Barea JM and Pearson VG.(eds). Mycorrhizas. Functional Process and Ecological Impact. Springer. Verlag Berlin Heidelberg, 33-45.

Rao NS. (1994). Soil Microorganisms and Plant Growth. Second Editions. University of Indonesia Publisher. Jakarta.