Comparative study of two isolation methods of Cyanobacteria in Côte d’Ivoire

Rosine Djeha Yao; Julien Kalpy Coulibaly; Mathias Ahoutou Koffi; Eric Kouamé Yao; Lydie Amon; René Kouamé Yao; Joseph Allico Djaman

doi:10.30574/gscbps.2019.8.1.0117

Authors

Rosine Djeha Yao Laboratory of Pharmacodynamic-Biochemistry, University Félix Houphouët-Boigny of Cocody, 22 BP 582 Abidjan 22, Côte d’Ivoire..
Julien Kalpy Coulibaly Department of Environment and Health / Unit of Chemistry and Environmental Microbiology, Pasteur Institute of Côte d’Ivoire , BP 490 Abidjan 22, Côte d’Ivoire.
Mathias Ahoutou Koffi Department of Environment and Health / Unit of Chemistry and Environmental Microbiology, Pasteur Institute of Côte d’Ivoire , BP 490 Abidjan 22, Côte d’Ivoire.
Eric Kouamé Yao Department of Environment and Health / Unit of Chemistry and Environmental Microbiology, Pasteur Institute of Côte d’Ivoire , BP 490 Abidjan 22, Côte d’Ivoire.
Lydie Amon Department of Environment and Health / Unit of Chemistry and Environmental Microbiology, Pasteur Institute of Côte d’Ivoire , BP 490 Abidjan 22, Côte d’Ivoire.
René Kouamé Yao Laboratory of Pharmacodynamic-Biochemistry, University Félix Houphouët-Boigny of Cocody, 22 BP 582 Abidjan 22, Côte d’Ivoire..
Joseph Allico Djaman Department of Clinical and Fundamental Biochemistry, Pasteur Institute of Côte d’Ivoire, BP 490 Abidjan 22, Côte d’Ivoire.

DOI:

https://doi.org/10.30574/gscbps.2019.8.1.0117

Keywords:

Aghien, Cyanobacteria, Isolation, Culture

Abstract

Cyanobacteria are prokaryotic organisms found in the freshwaters of most countries of the world. Interest in these organisms has increased in recent years because of their ability to produce toxins that are harmful to higher organisms. The objective of this study was to compare two methods of isolation of cyanobacteria in Côte d’Ivoire. Monthly water withdrawals were carried out from June 2016 to March 2018 on the Aghien Lagoon (Côte d'Ivoire) and analyzed using two methods, namely the isolation method on modified BG11 agar and the direct binocular method. A total of 1682 cyanobacteria were isolated, 1593 (94.70%) cyanobacteria were obtained by direct isolation and 89 (5.30%) by seeding isolation. The 1682 cyanobacteria were purified by successive transplanting on the agar BG11 modified, 563 cyanobacteria (33.47%) were obtained. Of this total, 92.36% (n = 520 cyanobacteria) were obtained by the direct method and 7.64% (n = 43 cyanobacteria) by the isolation method on modified BG11 agar. In order to maintain the growth of purified cyanobacteria, the 563 cyanobacteria were transferred to liquid modified BG11 medium. However 38 (6.74%) cyanobacteria had a positive growth on this medium of which 5.76% (n = 30) came from the direct method and 17.02% (n = 8) from the isolation method on modified BG11 agar. This study has shown that the isolation method that has resulted in better yield has been isolation by isolation method on modified BG11 agar.

Metrics

Metrics Loading ...

References

Pedro O, Correia D, Lie E, Skåre JU, Leã J, Neves L, Sandvik M and Berdal K. (2011). Polymerase chain reaction (PCR) detection of the predominant microcystine producing genotype of cyanobacteria in Mozambican lakes. African Journal of Biotechnology, 10 (83), 19299-19308.

Kononen K. (2001). Eutrophication, harmful algal blooms and species diversity in phytoplankton communities: Examples from the Baltic Sea. Ambio, 30 (4), 184-189.

Smith VH. (2003). Eutrophication of freshwater and coastal marine ecosystems. A global problem. Environmental Science and Pollution Research, 10 (2), 126-139.

Blais S. (2002). The issue of cyanobacteria (blue-green algae) at La Baie de Missisquoi in 2001. Agrosol, 13 (2), 103-110.

WHO. (2003). Algae and cyanobacteria in freshwater. In: Guidelines for safe recreational water environments. Volume 1: Coastal and fresh waters. World Health Organization, Geneva, (Switzerland), 136-158.

Azevedo SMFO, Carmichael WW, Jochimsen EM, Rinehart KL, Lau S, Shaw GR and Eaglesham G K. (2002). Human intoxication by microcystins during renal dialysis treatment in Caruaru. Brazil Toxicology, (181-182), 441-446.

Gugger M, Molica R, Le Berre, Dufour P, Bernard C and Humbert JF. (2005). Genetic diversity of Cylindrospermopsis strains (Cyanobacteria) isolated from four continents. Applied and Environmental Microbiology, 71 (2), 1970-1100.

Van Appeldoorn ME, Van Egmond HP, Speijers GJA and Bakker GJI. (2007). Toxins of cyanobacteria. Molecules Nutrition Food Ressources, 51 (1), 7-60.

Welker M and Von Döhren H. (2006). Cyanobacterial peptides. Nature’s own combinatorial biosynthesis. FEMS Microbiology Revues, 30 (4), 530-563.

Oufdou K and Oudra B. (2009). Bioactive substances developed by cyanobacteria isolated from certain Moroccan aquatic ecosystems. Africa Science, 5 (2), 260-279.

Coulibaly Kalpy J, Groga N, Soumahoro MK, Koudougou M, Ebrottié-Brou JE, Kouassi-Haoulley MT, Ouattara A, Faye-Ketté H and DOSSO M. (2014). Preliminary trial of implementation of cyanobacteria culture in Ivory Coast. International Journal of Biological Chemical Sciences, 8 (2), 551-565.

Rippka R, Waterbury J and Stanier R. (1981). Isolation and purification of cyanobacteria: Some general principles. In : Starr MP, Stops H, Triper HG, Balows A, Schelegel H G(Eds), The prokaryotes. Springer-Verlag, Berlin, 212-218.

Berger C. (2005). Cyanobacteria from the lower Senegal River Delta: Diversity, toxicity, toxins and associated risks. Thesis of the National Museum of Natural History of Paris, France, 144-145.

Mezouari S. (2017). Isolation and characterization of microorganisms of biotechnological interest in the region of Sidi Bel Abbes. Thesis of the Djillali Liabes University of Sidi Bel Abbes, Algeria, 35-36.

Meseck SL, Smith BC, Wikfors GH, Alix JH and Kapareiko D. (2007). Nutrient interactions between phytoplankton and bacterioplankton under different carbon dioxide regimes. Journal of Applied Phycology, 19 (3), 229-237.

Dakhama A, Noüe J and Lavoie M. (1993). Isolation and identification of antialgal substances produced Pseudomonas aeruginosa. Journal of Applied Phycology 5 (3), 297-306.

Le Chevanton M. (2013). Microalgae-bacteria interactions in bispecific experimental system: Effects on growth of Dunaliella sp. Thesis of the University of Nantes, France, 52-53.

Wilame R and Hoffman L. (2005). Cyanobacteria (blue algae) responsible for efflorescence in stagnant water. Toxicological and ecological aspects. Rapport final, 96-97.

Roger PA and Reynaud PA. (1977). Methods of isolation and purification of cyanophyceae. ORSTOM Papers, Biology Series, 10 (2), 121-128.

Gugger M and Couté A. (2006). Diversity and toxicity of cyanobacteria in water resources in Burkina Faso. Research Institute for Development UR167- CYROCO, 28-29.

Tassigny M and Lefebvre M. (1971). Self-hetero antagonism and other consequences of excretions of water or thermal algae. International Vereinigung für Limnologie, 19, 26-38.