Antibiotic susceptibility profile of bacteria isolated from packaged milk products sold in Sokoto, Nigeria: The resistant strains

Mudasiru Iyanda Omowale Raji; Mustapha Hussain Jiya

doi:10.30574/gscbps.2019.9.1.0179

Authors

Mudasiru Iyanda Omowale Raji Department of Pharmaceutics and Pharmaceutical Microbiology, Faculty of Pharmaceutical Sciences, Usmanu Danfodiyo University, Sokoto, Nigeria.
Mustapha Hussain Jiya Department of Pharmaceutics and Pharmaceutical Microbiology, Faculty of Pharmaceutical Sciences, Usmanu Danfodiyo University, Sokoto, Nigeria.

DOI:

https://doi.org/10.30574/gscbps.2019.9.1.0179

Keywords:

Antibiotic profile, Resistant strains, Milk products

Abstract

Antibiotics are generally the drug of choice in the treatment of various infections both in humans and animals. But the overuse and misuse of antibiotics in animal production and human medicine have led to the development of vast amount of resistant organisms difficult to treat with antibiotics and thereby posing serious health complications. This study was conducted to examine the antibiotic profile of bacteria isolated from packaged milk products sold in Sokoto metropolis in order to determine possibility of resistant strains. Forty-six bacteria from packaged milk products were subjected to antibiotics susceptibility test using a single disc of 7 antibiotics by disc diffusion method following Clinical and Laboratory Standards Institute’s guideline. Susceptibility result showed that high percentage of the bacteria were resistant to erythromycin (73.9%). However, very high percentages were susceptible to ciprofloxacin and tetracycline with 91.3% and 89.1% respectively. The finding of the study showed that packaged milk products sold in some areas in Sokoto metropolis contained resistant bacteria to commonly used antibiotics in the study area, therefore posing a serious health risk to the consumers. Good manufacturing practices need to be put in place to forestall any health problem as a result of consumption of contaminated packaged milk products.

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References

Carlet J, Collignon P, Goldmann D et al. (2011). Society’s failure to protect a precious resource: antibiotics. Lancet, 378, 369–71.

Conter V, Arico M, Valsecchi MG, Basso G, Biondi A, Madon E, Mandelli F, Paolucci G, Pession A, Rizzari C, Rondelli R, Zanesco L and Masera G. (2000). Long-term results of the Italian Association of Pediatric Hematology and Oncology (AIEOP) acute lymphoblastic leukemia studies, 1982–1995. Leukemia, 14, 2196– 2204.

Asai M, Iwata N, Yoshikawa A, Aizaki Y, Ishiura S, Saido TC and Maruyama K. (2007). Berberine alters the processing of Alzheimer’s amyloid precursor protein to decrease Abeta secretion. Biochem. Biophys. Res. Commun. 352, 498–502.

Gilchrist MJ, Greko C, Wallinga DB, Beran GW and Riley DG. (2007). The Potential Role of Concentrated Animal Feeding Operations in Infectious Disease Epidemics and Antibiotic Resistance. Environmental and Health Perspectives, 115, 313-316.

Threlfall EJ. (2002). Antimicrobial drug resistance in Salmonella: problems and perspectives in food and water-borne infections. FEMS Microbiology Reviews, 26, 141-148.

Emslie FR and Nel JR. (2002). An overview of the eradication of Brucella melitensis from KwaZulu-Natal. Onderstepoort J Vet Res, 69(2), 123 – 7.

FDA (Food and Drug Administration) (2012). Department of Human and Health Services. New animal drugs; cephalosporin drugs; extralabel animal drug use; order of prohibition. Final rule. Fed Reg, 77, 735–45.

CDC. (2011). Center for Disease Control.

WHO. (2012). World Health Organization. Critically important antimicrobials for human medicine. 3rd ed. Geneva, Switzerland.

Ungemach FR, Muller-Bahrdt D and Abraham G. (2006). Guidelines for prudent use of antimicrobials and their implications on antibiotic usage in veterinary medicine. International Journal of Medical Microbiology, 296(S2), 33 – 38.

NIAID. (2008).

McMahon KW, Fogel ML, Elsdon TS and Thorrold SR. (2010). Carbon isotope fractionation of amino acids in fish muscle reflects biosynthesis and isotopic routing from dietary protein. J. Anim. Ecol. 79(5), 1132–1141.

Byarugada DK. (2004). A view on antimicrobial resistance in developing countries and responsible risk factors. Int J Antimicrob Agents, 24(2), 105 – 10.

Okeke IN and Sosa A. (2003). Antibiotic resistance in Africa – discerning the enemy and plotting a defence. Africa Health, 10-15.

Nys S, Okeke IN, Kariuki S, Dinant GJ, Driessen C and Stobberingh EE. (2004). Antimicrobial resistance of fecal E. coli from healthy volunteers from eight developing countries. Journal of Antimicrobial Chemotherapy, 54(5), 952-955.

WHO (‎2000)‎. The World health report: 2000: Health systems: improving performance. World Health Organization.

WHO. (2002). World health report 2002. Reducing risks, promoting healthy life. Geneva, Switzerland.

Okeke IN, Laxminarayan R, Bhutta ZA, Duse AG, Jenkins P, O'Brien TF, Pablos-Mendez A and Klugman KP. (2005). Antimicrobial resistance in developing countries. Part I: recent trends and current status. Lancet Infect Dis., 5(8), 481-93.

Gould I. (2004). Risk Factors for Acquisition. European Journal of Clinical Microbial Infectious Diseases, 30-38.

Hove A, Garella J and Genzini D. (2001). Methods of yogurt production. Journal of Dairy and Food Engineering, 5-8.

Chui C, Wu T, Su L, Cu C, Chia J, Kuo A et al. (2002). The emergence in Taiwan of fluoroquinelone resistance in Salmonella enteric serotype Cholerasuls. The New England Journal of Medicine, 416-419.

Teka G. (1997). Food Hygiene Principles and Food Borne Disease Control with Special Reference to Ethiopia. 1st Ed, Faculty of Medicine, Department of Community Health, Addis Ababa University, Ethiopia, 73-86.

CLSI (2013). Performance standard for antimicrobial susceptibility testing. Approved standard M31-A3. 3rd edition. Vol. 26 (3), 32- 35.

Jorgensen JH and Turnidge JP. (2007). Susceptibility test methods: dilution and disk diffusion methods. In: Murray PR, Baron EJ, Jorgensen JH, Landry ML and Pfaller MA, Editors. Manual of Clinical Microbiology. 9th edition. ASM press, Washington, DC, USA, 1152–1172.

WHO (2013). World Health Organization. Antimicrobial Resistance: Global Report on Surveillance, Geneva.

Leibowitz HM. (1991a). Antibacterial effectiveness of ciprofloxacin 0.3% ophthalmic solution in the treatment of bacterial conjunctivitis. Am J. Ophthalmol, 112(4 suppl), 29S – 33S.

Leibowitz HM. (1991b) Clinical evaluation of ciprofloxacin 0.3% ophthalmic solution for treatment of bacterial keratitis. Am J. Ophthalmol, 112(4 suppl), 34S – 47S.

Okonko IO, Soleye FA, Amusan TA, Ogun AA, Ogunnusi TA and Ejembi J. (2009). Incidence of Multi-Drug Resistance (MDR) Organisms in Abeokuta, Southwestern Nigeria. Global Journal of Pharmacology, 3(2), 69-80.

Ayandiran TO, Falgenhauer L, Schmiede J, Chakraborty T and Ayeni FA. (2018). High resistance to tetracycline and ciprofloxacin in bacteria isolated from poultry farms in Ibadan, Nigeria. J Infect Dev Ctries, 12(6), 462 – 470.

Hostetler KA and Thurman EM. (1999). Determination of ionic chloroacetanilide herbicide metabolites in surface water and ground water by high-performance liquid chromatography-diode array detection and high-performance liquid chromatography/mass spectrometry. In: Morganwalp DW and Buxton HT, Eds., U.S. Geological Survey Toxic Substances Hydrology Program—Proceedings of the Technical Meeting, Charleston, South Carolina, Volume 2— Contamination of Hydrologic Systems and Related Ecosystems: U.S. Geological Survey Water-Resources Investigations Report 99–4018B.