In vitro propagation of Satureja thymbra L. (Lamiaceae): A valuable aromatic-medicinal native plant of the Mediterranean region

Sarropoulou Virginia; Maloupa Eleni

doi:10.30574/gscbps.2019.9.2.0190

Authors

Sarropoulou Virginia Laboratory of Protection and Evaluation of Native and Floricultural Species, Balkan Botanic Garden of Kroussia, Institute of Plant Breeding and Genetic Resources, Hellenic Agricultural Organization (HAO) – DEMETER, P.O. Box 60458, P.C. 570 01, Thermi, Thessaloniki, Greece.
Maloupa Eleni Laboratory of Protection and Evaluation of Native and Floricultural Species, Balkan Botanic Garden of Kroussia, Institute of Plant Breeding and Genetic Resources, Hellenic Agricultural Organization (HAO) – DEMETER, P.O. Box 60458, P.C. 570 01, Thermi, Thessaloniki, Greece.

DOI:

https://doi.org/10.30574/gscbps.2019.9.2.0190

Keywords:

Ex situ conservation, Mediterranean endemic, Micropropagation, Plant growth regulators, Satureja thymbra, Tissue culture media

Abstract

Satureja thymbra L. or savory is a highly threatened aromatic-medicinal endemic Mediterranean plant. Micropropagation can play a role in the protection of the natural ecosystem. The disinfection protocol involved the immersion of shoot-tip explants in 70% alcohol (1 min) and 3% NaOCl (15 min) giving 61.18% success. The MS medium supplemented with 30 g/l sucrose and 7 g/l Plant Agar was optimum for the initial establishment stage (10-day culture, 92.31% proliferation) and afterwards supplemented extra with 0.5 mg/l BA for multiple shoot induction (5 weeks) (100% proliferation, 5.5 shoots 14.24 mm long). In rooting stage, 1 mg/l IBA gave the highest rooting (58.62%), 0.5 mg/l NAA the maximum root number (12.13) and 0.1 mg/l IAA the largest root length (34.35 mm) (MS medium + 20 g/l sucrose + 6.5 g/l Plant Agar, 7 weeks) (Experiment No. 1). There was a progressive increase in rooting and shoot proliferation increasing the number of subcultures, being highest (100% rooting, 6 roots 70 mm long, 38.1% shoot multiplication, 2 shoots 30 mm long) in the 4^th subculture/ 4.5 weeks each in WPM medium (Experiment No. 2). Therefore, best rooting results (50-100%) were accomplished in 3 different composition media: (1 and 2) WPM medium + 20 g/l sucrose + 6 g/l Plant Agar (1^st and 4^th subculture) and (3) MS medium + 30 g/l sucrose + 1 mg/l IBA + 7 g/l Plant Agar. The ex vitro survival of rooted microshoots after 4 weeks in the greenhouse mist (1 peat moss: 1 perlite) was 81.82%.

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References

Ruffoni B, Pistelli L, Bertoli A and Pistelli L. (2010). Plant cell cultures: Bioreactors for industrial production. Advances in Experimental Medicine and Biology, 698, 203-221.

Lucchesini M and Mensuali A. (2010). Plant tissue culture - An opportunity for the production of nutraceuticals. Advances in Experimental Medicine and Biology, 698, 185-202.

Pistelli L, Giovannini A, Ruffoni B, Bertoli A and Pistelli L. (2010). Hairy root cultures for secondary metabolites production. Advances in Experimental Medicine and Biology, 698, 167-184.

Canter PH, Thomas T and Ernst E. (2006). Bringing medicinal plants into cultivation: opportunities and challenges for biotechnology. Trends in Biotechnology, 23, 180-185.

Debnath M, Malik CP and Bisen PS. (2006). Micropropagation: a tool for the production of high quality plant-based medicines. Current Pharmaceutical Biotechnology, 7(1), 33-49.

Zhou LG and Wu JY. (2006). Development and application of medicinal plant tissue cultures for production of drugs and herbal medicinals in China. Natural Product Reports, 23(5), 789-810.

Gupta AK, Harish Rai MK, Phulwaria M, Agarwal T and Shekhawat NS. (2014). In vitro propagation, encapsulation, and genetic fidelity analysis of Terminalia arjuna: A cardioprotective medicinal tree. Applied Biochemistry and Biotechnology, 173(6), 1481-1494.

Monemi MB, Kazemitabar SK, Khaniki GB, Yasari E, Sohrevardi F and Pourbagher R. (2014). Tissue culture study of the medicinal plant leek (Allium ampeloprasum L). International Journal of Molecular and Cellular Medicine, 3, 118-125.

Sefidkon F and Jamzad Z. (2005). Chemical composition of the essential oil of three Iranian Satureja species (S. mutica, S. macrantha and S. intermedia). Food Chemistry, 91, 1-4.

Satil F and Kaya A. (2007). Leaf anatomy and hairs of Turkish Satureja L. (Lamiaceae). Acta Biologica Cracoviensia series Botanica, 49, 67-76.

Tariku Y, Hymete A, Hailu A and Rohloff J. (2010). Essential-oil composition, anti-leishmanial, and toxicity study of Artemisia abyssinica and Satureja punctata ssp. punctata from Ethiopia. Chemistry & Biodiversity, 7, 1009-1018.

Mouterde P. (1983). Nouvelle flore du Libanet de la Syrie, volume 3. Dar El-Mashreq Editeurs, Beirut, Lebanon, 578.

Greuter W, Burdet HM and Long G. (1986). Med-Checklist. Editions des Conservatoire et Jardin Botanique de la ville de Geneve, 3, 313.

El Beyrouthy M. (2008). Contribution à l'ethnopharmacologie libanaiseet aux Lamiaceaedu Liban. Acta Botanica Gallica, 156(3), 515-521.

El Beyrouthy M, Arnold N, Delelis A and Dupont F. (2008). Plants used as remedies antirheumatic and antineuralgic in the traditional medicine of Lebanon. Journal of Ethnopharmacology, 120(3), 315-334.

Capone W, Mascia C, Spanedda L and Chiappini M. (1989). Chemical composition and antibacterial activity of the essential oil from Sardinian Satureja thymbra. Fitoterapia, 60, 90-92.

Kokkini S and Vokou D. (1989). Carvacrol rich plants in Greece. Flavour and Fragrance Journal, 4, 1-7.

Dimopoulos P, Raus T, Bergmeier E, Constantinidis T, Iatrou G, Kokkini S, Strid A and Tzanoudakis D. (2013). Vascular plants of Greece: An annotated checklist. Berlin: Botanic Garden and Botanical Museum Berlin-Dahlem & Athens: Hellenic Botanical Society. Englera, 31, 1-370.

Dimopoulos P, Raus T, Bergmeier E, Constantinidis T, Iatrou G, Kokkini S, Strid A and Tzanoudakis D. (2016). Vascular plants of Greece. Αn annotated checklist. Supplement. Willdenowia, 46, 301-347.

Arrebola ML, Socorro O, Barcelò-Muńoz A, Simòn-Pérez E and Pliego-Alfaro F. (1997). Micropropagation of Satureja obovata Lag. HortScience, 32(7), 1278-1280.

Karimi N, Ghasmpour HR and Yari M. (2014). Effect of different growth regulators on callus induction and plant regeneration of Satureja species. Annual Research & Review in Biology, 16, 2646-2654.

Murashige T and Skoog F. (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum, 15, 473-493.

Lloyd G and McCown BH. (1980). Commercially-feasible micropropagation of Mountain Laurel, Kalmia latifolia, by shoot tip culture. Proceedings of the International Plant Propagator's Society, 30, 421-427.

Teshome S and Soromessa T. (2015). In vitro propagation of Satureja abyssinica (Benth.) Briq. - A valuable medicinal plant. Advanced Life Science and Technology, 34, 100-109.

Teshome S, Soromessa T and Feyissa T. (2017). In vitro propagation of a threatened medicinal plant Satureja abyssinica through nodal explants - Antimicrobial and antifungal herb. International Journal of Bio Sciences and Technology, 10(3), 20-25.

Hwang I, Sheen J and Müller B. (2012). Cytokinin signaling networks. Annual Review of Plant Biology, 63, 353-380.

Sharma RK and Wakhlu AK. (2003). Regeneration of Heracleum candicans Wall plants from callus cultures through organogenesis. Journal of Plant Biochemistry and Biotechnology, 12, 71-72.

Ramak P, Sharifi M, Osaloo SK, Ebrahimzadeh H and Behmanesh M. (2011). Studies on seed germination and in vitro shoot multiplication of Satureja khuzistanica Jamzad, an important medicinal plant. African Journal of Biotechnology, 10(83), 19407-19414.

Linsmaier EM and Skoog F. (1965). Organic growth factor requirements of tobacco tissue culture. Physiologia Plantarum, 18, 100-127.

Teshome I, Teshome S, Soromessa T and Feyissa T. (2016). Development of an efficient in vitro propagation protocol for Satureja punctata - A rare aromatic and medicinal plant. Taiwania, 61(1), 41-48.