Antioxidant, antibacterial activities and phytochemical screening of Asteriscus pygmaeus aerial parts ethanolic extract

Authors

  • Nabila Helmy Shafik Chemistry of Tanning Materials and Leather Technology Department, National Research Centre, Dokki, Cairo, Egypt.
  • Reham Ezzat Shafek Chemistry of Tanning Materials and Leather Technology Department, National Research Centre, Dokki, Cairo, Egypt.
  • Helana Naguib Michael Chemistry of Tanning Materials and Leather Technology Department, National Research Centre, Dokki, Cairo, Egypt.

DOI:

https://doi.org/10.30574/gscbps.2019.9.3.0218

Keywords:

Asteraceae, Asteriscus pygmaeus, flavonoids, Antioxidant, Antibacterial activities

Abstract

Phytochemical investigation of the Aerial parts of Asteriscus pygmaeus ethanolic extract gave rise to isolation of a new compound: 6-Hydroxykaempferol 6-methylether (eupafolin) -4' glucopyranoside (1) together with eight known metabolites; patuletin 7-glucoside (2), chrysoeriol 7- O- glucoside (3), 6-Hydroxy kaempferol (4), 6-Hydroxy kaempferol 6-methyl ether (eupafolin) (5), 6,4` dimethoxy luteolin (6), patuletin (7), chrysoeriol (8) and luteolin (9). Their structures were established by chromatographic methods, chemical degradation and various spectroscopic data; compared with previously known analogues. Antioxidant activity of ethanolic extract was investigated using DPPH (2,2-diphenyl-1-picrylhydrazyl) as stable radicals method indicated a high radical scavenging activity for it. The results for antimicrobial study for extract against bacteria and fungi showed that it exhibited broad spectrum of antimicrobial activity against gram positive bacteria, gram negative bacterial and fungal cultures.

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References

Tepe B, Daferera D, Sokmen A, Sokmen M and Polissiou M. (2005). Antimicrobial and antioxidant activities of essential oil and various extracts of salvia tomentosa miller (lamiaceae). Food Chem, 90, 333–340.

Khanna D, Sethi G, Ahn KS, Pandey MK, Kunnumakkara AB, Sung B, Aggarwal A and Aggarwal BB. (2007). Natural products as a gold mine for arthritis treatment. Curr Opin Pharmacol,7(3), 344–351.

Fusco D, Colloca G, Lo Monaco MR and Cesari M. (2007). Effects of antioxidant supplementation on the aging process. J Clin Interv Aging, 2, 377–387.

Loizzo MR, Tundis R,Conforti F, Saab AM, Statti GA and Menichini F. (2007). Composition and α-amylase inhibitory effect of essential oils from cedrus libani. Fitoterapia, 78, 323–326.

Cristofari G, Znini M, Majidi L, Mazouz H, omi P, Costa J and Paolini J. (2012). Chemical Diversity of Essential Oils from Asteriscus graveolens (Forssk.) Less: Identification of cis‐8‐Acetoxychrysanthenyl Acetate as a New Natural Component, Chemistry & Biodiversity, 9, 727- 738.

Ezzat MI, Ezzat SM, El Fishawy AM, El Deeb KS and El Toumy SA. (2016). A new acylated flavonol from the aerial parts of Asteriscus maritimus (L.) Less (Asteraceae), Journal Natural Product Research, 30, 1753–1761.

Ahmed AA, Ishak M S, Micheal H N, El-Ansari M A and El-Sissi H I.(1991). Flavonoids of Asteriscus graveolens, J. Nat. Prod, 54 (4), 1092–1093.

Dawidar AM, Ayyad SN, Abdel-Mogib M and Alarif WM. (2005). Humulene Sesquiterpene Lactones from Asteriscus schimperi, J. saudi chem. soc, 9, 137-142.

Akssira M, Mellouki F, Salhi A, Alilou H, Saouf A, El Hanbali F, Arteaga JF and Barrero AF. (2006). Naupliolide, a sesquiterpene lactone with a novel tetracyclic skeleton from Nauplius graveolens susp odorus, Tetrahedron Lett. 47, 6719–6721.

Ramdanea F, Essid R, Mkadmini K, Hammami M, Fares N, Mahammed MH, El Ouassis D, Tabbene O, Limam F and Hadj MDO. (2017). Phytochemical composition and biological activities of Asteriscus graveolens (Forssk) extracts, Process Biochemistry, 56, 186–192.

Benchelah AC, Bouziane H and Maka M. (2004). Fleurs du Sahara, arbres et arbustes, voyage au cœur de leurs usages avec les Touaregs du Tassili, Phytotherapie, 2, 191-197.

Boussaada O, Chriaa J, Nabli R, Ammar S, Saidana D, Mahjoub MA, Chraeif I, Helal AN and Mighr Z. (2008). Antimicrobial and antioxidant activities of methanol extracts of Evax pygmaea (Asteraceae) growing wild in Tunisia. World Journal of Microbiology and Biotechnology, 24 (8), 1289–1296.

Mativandlela SPN, Lall N and Meyer JJN. (2006). Antibacterial, antifungal and antitubercular activity of (the roots of) Pelargonium reniforme (CURT) and Pelargonium sidoides (DC) (Geraniaceae) root extracts. South African Journal of Botany,72, 232–237.

Askarne L, Talibi I, Boubaker H, Boudyach EH, Msanda F, Saadi B and Ben Aoumar AA. (2013). Use of Moroccan medicinal plant extracts as botanical fungicide against citrus blue mould, Lett. Appl. Microbiol, 56(1), 37–43.

Sarg TM, El-Dahmy SI, Ateya AM and Abdel-Fattah HA. (1994). Two new bisabolone hydroperoxides and biological activity of Asteriscus graveolens ,Fitoterapia, 65, 241-244.

Saraswathi J, Venkatesh K, Baburao N, Hilal MH and Roja Rani A. (2011). Phytopharmacological importance of pelargonium species. Journal of Medicinal Plants Research, 5(2), 587–2598.

Gamez E JC, Luyengi L, Lee SK, Zhu LF, Zhou HBN, Fong HS, Pezzuto JM and Kinghorn AD. (1998). Antioxidant flavonoid glycosides from Daphniphyllum calycinum, J. Nat. Prod., 61, 706-708.

Kumar G, Karthik L and Rao KVB. (2010). Phytochemical composition and in vitro antimicrobial activity of Bauhinia racemosa LAMK (caesalpinaceae), Int J Pharmaceutical Sciences Review and Research, 1(11), 51-58.

Ajay Ka, Lokanatha Rai and Umesha KB. (2002). Evaluation of antibacterial activity of 3,5-dicyano-4,6-diaryl-4-ethoxycarbonyl-piperid-2-ones, J Pharm Biomed Anal, 27(5), 837-840.

Kumar G, Karthik L and Rao KVB. (2010). Antibacterial activity of aqueous extract of Calotropis gigantea leaves - An in vitro study, Int J Pharmaceutical Sciences Review and Research, 4(2), 141-144.

Smith DM, Glennie CW and Harborne JB. (1971). Identification of eupalitin, eupatolitin and patuletin glycosides in ipomopsis aggregata, Phytochemistry, 10, 3115-3120.

Harborne JB. (1993).The Flavonoids Advances in Research Since 1986. In: Markham KR, Geiger H (eds.). 1H nuclear magnetic resonance spectroscopy of flavonoids and their glycosides in hexadeutero dimethyl sulfoxide, Chapman and Hall, London, 441- 497.

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Published

2019-12-30

How to Cite

Shafik, . N. H., Shafek, . R. E., & Michael, H. N. (2019). Antioxidant, antibacterial activities and phytochemical screening of Asteriscus pygmaeus aerial parts ethanolic extract. GSC Biological and Pharmaceutical Sciences, 9(3), 041–046. https://doi.org/10.30574/gscbps.2019.9.3.0218

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