Chemo-profiling and assessment of antioxidant activity and antibacterial potentials of selected plants of family Combretaceae
DOI:
https://doi.org/10.30574/gscbps.2020.10.2.0016Keywords:
Antibacterial assay, Antioxidant, Chemo-profiling, Combretum species. HPTLC, Terminalia spp.Abstract
Traditional medicine is used all over the world, especially in developing countries. Though synthetic drugs are very effective but are harmful to human beings because of their side effects. Genus of Terminalia and Combretum species belongs to the family Combretaceae, which contains 20 genera and 600 species as herbs, shrubs, and trees. These plants are used in different traditional medicine for their antioxidant property and also for the treatment of hepatitis and malaria due to the presence of antibacterial properties. The present study is focused on chemo- profiling, screening of the antioxidant compound, antioxidant activities and antimicrobial properties of different Terminalia and Combretum species, namely Terminalia arjuna, T. alata, T. bellerica, T. T. cataappa, T. chebulla, T. bucera and T. mauritiana,, Combretum albidum, C. roxburghii and C. indicum. Four different concentrations (20, 40, 60 and 80 mg/ml) of methanolic extracts of leave and bark of these plant species were tested against six bacterial pathogens i.e Streptococcus epidermis, Salmonella enterica, Staphylococcus aureus, Escherichia coli, Bacillus subtillis and Pseudomonas aeruginosa using disc diffusion method. It was observed that the methanolic leaf and bark extract T. arjuna, T. chebulla, and C. albidum proved remarkable antibacterial properties against the different bacterial strains. This study will help to know the properties of medicinal plants for making different formulation of different types of green medicine.
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References
Martini ND, Katerere DRP and Eloff JN. (2004). Biological activity of five antibacterial flavonoids from Combretum erythrophyllum (Combretaceae). Jour. of Ethnopharmacology, 93,207-212.
Richardson MA. (2001). Bio pharmacologic and herbal therapies for cancer: research update from National Centre for complementary and alternative medicine, Journal of Nutrition, 131(11), 3037S–3040S.
Farnsworth NR and Soejarto DD. (1991).Global importance of medicinal plants. In: Akerele O., Heywood V. and Synge H. (eds) The conservation of medicinal plants. Cambridge University Press, Cambridge, UK, 25-51.
Verpoorte R. (2000). Pharmacognosy in the New Millenium: Lead finding and biotechnology, Journal of Pharmacy and Pharmacology, 52, 253-262.
SowemimoAA, Van de Venter M, Baatjies L and Koekemoer T. (2009). Cytotoxic activity of selected Nigerian plants. African Journal of Traditional and Alternative Medicines. 6(4), 526-528.
Bhatnagar B, Sahoo S, Mohapatra AK and Behera DR. (2012). Phytochemical analysis, Antioxidant and Cytotoxic activity of medicinal plant Combretum roxburghii (Family: Combretaceae), Int. Journal. Drug Dev. & Res., 4(1), 193-202.
Chandel SR, Kumar V, Guleria S, Sharma N, Sourirajan A, Khosla PK, Baumler DJ and Dev K. (2019). Sequential Fractionation by Organic Solvents Enhances the Antioxidant and Antibacterial Activity of Ethanolic Extracts of Fruits and Leaves of Terminalia bellerica from North Western Himalayas, India. Pharmacog. Journal, 11(1), 94-101.
Beigi M, Haghani E, Alizadeh A and Samani ZN. (2018). The pharmacological properties of several species of Terminalia in the world. International Journal of Pharmaceutical Sciences and Research, 9(10), 4079-4088.
Pettit GR, Singh SB, Hamel E, Lin CM,Alberts DS and Garcia-Kendall D. (1989). Isolation and structure of the strong cell growth and tubulin inhibitor combretastatin A-4. Experientia, 45(2), 209–211.
Pettit GR, Singh SB, Schmidt JM, Niven ML, Hamel E and Lin CM. (1988). Isolation, structure, synthesis, and antimitotic properties of combretastatins B-3 and B-4 from Combretum caffrum. Journal of Natural Products, 51(3), 517- 527.
Abera B. (2014). Medicinal plants used in traditional medicine by Oromo people, Ghimbi District, Southwest Ethiopia. Journal of Ethnobiology and Ethnomedicine, 10, 40.
Stahl E. (1969). Thin Layer Chromatography. 2nd Edn Springer-Verlag,New York.
Das A, Samal KC, Das AB and Rout GR. (2018). Quantification, Antibacterial Assay and Cytotoxic Effect of Combretastatin, an Anticancer Compound from Three Indian Combretum species. Int. J. Curr.Microbiol.App.Sci., 7, 687-699.
Deby C and Magotteaux G. (1970). Relationship between essential fatty acids and tissue antioxidant levels in mice. SocBiolFil., 164(12),2675-2681.
Lowry OH, Rosenbrough NJ, Farr AL and Randall RJ. (1951). Protein measurement with theFolin Phenol Reagent. Journal of Biological Chemistry, 193,265-275.
Beauchamp C and Fridovich I. (1971). Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. Analytical Biochemistry, 44, 276-287.
Abei H. (1984). Catalase in vitro. Methods in Enzymology. Science Direct, Elsevier, 105, 121- 126.
Cock IE. (2015).The medicinal properties and phytochemistry of plants of the genus Terminalia (Combretaceae). Inflammopharmacology, 23(5), 203–229.
Kolla JN, Kulkarni N, Kura RR and Theepireddy SKR. (2017). Terminalia chebula Retz.–an important medicinal plant. HerbaPolorica, 63(4), 45-56.
Das N, Goshwami D, Md. Hasan S, Mahmud ZAL and Raihan SZ. (2016). Evaluation of antioxidant, antimicrobial and cytotoxic activities of Terminalia citrine leaves. Journal of Pharmacy Research, 10(1), 8-15.
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