Safety and health benefits profile studies of leaf extracts of Momordica balsamina Linn (Cucurbitaceae) found in North Central Nigeria

Okai Jonathan Kadiri; Sylvester Ignatius Okafor; Joshua Ikoni Ogaji

doi:10.30574/gscbps.2020.11.2.0039

Authors

Okai Jonathan Kadiri Department of Pharmaceutical Technology and Industrial Pharmacy, Faculty of Pharmaceutical Sciences, University of Jos, Jos, Nigeri.
Sylvester Ignatius Okafor Department of Pharmaceutical Technology and Industrial Pharmacy, Faculty of Pharmaceutical Sciences, University of Jos, Jos, Nigeri.
Joshua Ikoni Ogaji Department of Pharmaceutical Technology and Industrial Pharmacy, Faculty of Pharmaceutical Sciences, University of Jos, Jos, Nigeri.

DOI:

https://doi.org/10.30574/gscbps.2020.11.2.0039

Keywords:

Momordica balsamina, Composition, Radical scavenging, Safety, Health

Abstract

Momordica balsamina Linn (Cucurbitaceae) plant possesses both medicinal and nutritional properties and has been used for its nutritional and medicinal benefits. The purpose of this paper was to evaluate the safety and health benefits of the leaf extracts of the plant found in North Central Nigeria. The leaf powder was sequentially extracted with solvents of varying polarity index. The protein and amino acid contents were determined by micro Kjeldahl nitrogen methods. The lipid, carbohydrate, fibre and ash contents were determined by well-established and standardized methods. The mineral compositions were analyzed by atomic absorption spectrophotometer. The antioxidant activity was evaluated by the scavenging activity of the extracts on DPPH free radical, while the acute toxicity studies were carried out using male albino rats. Results show that the leaf contains 22 ± 0.58% of crude proteins on dry weight basis. The corresponding values for carbohydrates, lipids, crude fibre and ash are 32.5 ± 0.8, 3.98 ± 0.12, 16.72 ± 1 and 21 ± 0.81% respectively. The calorific value and moisture content are 201.23 Kcal /100 g and 3.77 ± 0.22 % respectively. The leaf was composed of macro- and micro nutrients. The methanolic extract possesses the highest radical scavenging activity. The median lethal dose of the aqueous extract at 5000 mg/kg body weight showed no mortality. These results indicate that the leaf of Momordica balsamina possesses nutritional and antioxidant properties, and is safe for consumption as herbal vegetable.

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References

Ekor M. (2014). The growing use of herbal medicine: issues relating to adverse reactions and challenges in monitoring safety. Frontiers in Pharmacology, 4 (177), 177.

Yang W. (2014).Botanical, pharmacological, phytochemical, and toxicological aspects of antidiabetic plant Bidens pilosa L. Evidence-based Complimentary and Alternative Medicine, 1-14.

Dalei J, Rao VM, Sahoo D, Rukmini M and Ray R. (2016). Review on the nutritional and pharmacological potencies of Moringaoleifera. European Journal of Pharmaceutical and Medical Research, 3(1), 150-155.

Kadiri O and Olawoye B. (2016).Vernonia amygdalina: an underutilized vegetable with nutriceutical potentials- a review. Turkish Journal of Agriculture- Food Science and Technology, 4(9), 763 – 768.

Khaw K, Bingham S, Welch A, Luben R, Wareham N, Oakes S and Day N. (2001). Relation between plasma ascorbic acidand mortality in men and women in EPIC-Norfolk prospective study:a prospective population study. The Lancet, 357, 657–663.

Thakur GS, Pandey M, Sharma R, Sanodiya BS, Prasad GBKS and Bisen PS. (2011). Factors affecting in vitro propagation of Momordica balsamina: a medicinal and nutritional climber. Physiology and Molecular Biology of Plants, 1(2), 193 – 197.

Kaur I, Puri M, Ahmed Z, Blanchet FP, Mangeat B and Piguet V. (2013).Inhibition of HIV1 Replication by Balsamin, a Ribosome Inactivating Protein of Momordica balsamina .PLoS ONE, 8(9), 1 -9.

Mshelia SH and Kurami Y. (2017). Anti-ulcerogenic effect of extracts of Momordica balsamina Linn against experimentally induced gastric ulceration in rats. International Journal of Medicinal plants Research, 6(4), 332 – 336.

Akinyemi KO, Mendie UE, Smith ST, Oyelolu AO and Coker AO. (2005). Screening of some medicinal plants for anti-salmonella activity, Journal of Herbal Pharmacotherapy, 5(1), 45-60.

Hassan LG and Umar K.J. (2006) Nutritional value of balsam apple leaves. Pakistan Journal Nutrition,5, 522–529.

Jigam AA, Akanya HO and Adeyemi DJ. (2004).Antimicrobial and antiplasmodial effects of Momordica balsamina. Nigeria Journal of Natural Product Medicine, 8, 11–12.

Kadiri JO, Okafor IS and Ogaji IJ. (2020). Effect of extraction solvents on the phytochemical constituents and antibacterial property of leaf extracts of Momordica balsamina Linn (Cucurbitaceae) found in North Central Nigeria. International Journal of Current Trends in Pharmaceutical Research, 8(1), 6 – 12.

Ekeanyanwu C. (2013). Evaluation of crude protein and amino acid composition of Nigerian Mondora myristica (Ehuru).Parkistan Journal of Nutrition, 12(3), 219 – 223.

Food and Agriculture Organisation (FAO) of the United Nations Rome (2003). Food energy- methods of analysis and conversion factors. FAO Food and Nutrition paper, 77

Yust MM, Pedroche J, Girόn-Calle J and Alaiz M. (2004). Determination of tryptophan by high performance liquid chromatographic detection. Food Chemistry, 85(2), 317 – 320.

Brand-Williams W, Cuvelier ME and Berset C. (1995). Use of a free radical method to evaluate antioxidant activity. LWT- Food Science and Technology, 28 (1), 25 – 30.

Deora SP, Mishra KC, Mavani P, Asha R, Shrivastava B and Rajesh NK. (2010). Effective alternative methods of LD50 help to save number of experimental animals. Journal of Chemical and Pharmaceutical Research, 2(6), 450 – 453.

Parasuraman S. (2011).Toxicological screening.Journal of Pharmacology and Pharmacotherapy, 2(2), 74 – 79.

Flyman MV and Afolayan A J. (2009). Proximate and mineral composition of the leaves of Momordica balsamina L: An underutilized wild vegetable in Botswana. International Journal of Food Science and Technology, 58(6), 419 – 423.

Pereira MA, O’Reilly E and Augustsson K. (2004). Dietary fibre and risk of coronary heart disease. A pooled analysis of cohort studies. Archnite Internal Medicine, 164 (4), 370 – 376.

Galano A and Alvarez-Idaboy JR. (2011). Glutathione: Mechanism and kinetics of its non-enzymatic defense action against free radicals. Royal Society of Chemistry Advances, 1(9), 1763 – 1771.

Kunle FO and Egharevba OH. (2013). Chemical Constituents and Biological activity of medicinal plants used for the Management of Sickle cell Disease – A review. Academic Journals, 7 (8), 3452-3476.

Castiglioni S, Cazzaniga A, Albisetti W and Maier JAM. (2013). Magnesium and osteoporosis: current state of knowledge and future research directions. Nutrients, 5 (8), 3022 – 3033.

Perez V and Chang ET. (2014). Sodium – to – potassium ratio and blood pressure, hypertension, and related factors. Advances in Nutrition, 5, 712 – 741.

Houston MC and Harper KJ. (2008). Potassium, magnesium and calcium: their role in both the cause and treatment of hypertension. The Journal of Clinical Hypertension, 10 (7), 2 – 11.

Koh ES, Kim SJ, Yoon HE, Chung JH, Chung S, Part CW and Shin SJ. (2014). Association of blood manganese level with diabetes and renal dysfunction: a cross-sectioned study of the Korean general population. BMC Endocrine Disorders, 14 (24), 1 – 8.

Osredkar J and Sustar N. (2011). Copper and zinc, biological role and significance of copper/zinc imbalance. Journal of clinical Toxicology, 3 (1),1- 18.

FitrianaWD,Ersam T,Shimizu K and Fatmawati S. (2016). Antioxidant activity of Moringa oleifera extracts. Indonesian Journal of Chemistry, 1 (3), 297 – 301.

Jiang Z, Chen Y, Yoa F, Chen W, Zhong S, Zheng F and Shi G. (2013). Antioxidant, antibacterial and antischistosomal activities of extracts from Grateloupa livida (Harv) yamada. PLOS ONE, 2(1), 181 – 186.

Michalak A. (2006). Phenolic compounds and their antioxidant activity in plants growing under heavy metal stress. Polish Journal of Environmental Studies, 15(4), 523 – 530.

Sengul M, Yildiz H, Gungor N, Cetin B, Eser Z and Ercisli S. (2009). Total phenolic content, antioxidant and antimicrobial activities of some medicinal plants. Pakistan Journal of Pharmaceutical Sciences, 22(1), 102 – 106.

Kurami Y, Onyeyili PO and Ogubuaja VO. (2006). Toxicity studies and effects of Momordica balsamina (Balsam apple) aqueous extract on serum electrolytes and plasma trace elements. Sahel Journal of Veterinary Sciences, 5 (1), 13 – 19.

Ahmed M. (2015). Acute toxicity (lethal dose 50 calculation of herbal drug somina in rats and mice), Pharmacology and Pharmacy, 6(3), 185 – 189.